succiniclasticum_medium

CultureMech:001805 bacterial COMPLEX LIQUID
Original name: SUCCINICLASTICUM MEDIUM

Overview

External ontology
mediadive.medium:666 (source link unavailable) — SUCCINICLASTICUM MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 6.7, 'max': 6.8}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium666.pdf

Composition

flowchart LR
    CultureMech_001805["succiniclasticum_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_001805 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001805 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NaCl["NaCl"]
    CultureMech_001805 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001805 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001805 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001805 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_Indigocarmine["Indigocarmine"]
    CultureMech_001805 --> ing6_Indigocarmine
    CHEBI_31695["CHEBI:31695"]
    ing6_Indigocarmine -.-> CHEBI_31695
    ing7_NaHCO3["NaHCO3"]
    CultureMech_001805 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_Disodium_succinate["Disodium succinate"]
    CultureMech_001805 --> ing8_Disodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing8_Disodium_succinate -.-> CHEBI_63675
    ing9_Yeast_extract["Yeast extract"]
    CultureMech_001805 --> ing9_Yeast_extract
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001805 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001805 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
0.23 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.23 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NaCl
Cl.Na 58.443 g/mol
0.45 G_PER_L CHEBI:26710
sodium chloride
4 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.45 G_PER_L CHEBI:62946
ammonium sulfate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.06 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.09 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 Indigocarmine
C16H8N2O8S2.2Na 466.36 g/mol
0.005 G_PER_L CHEBI:31695
indigo carmine
8 NaHCO3
CHO3.Na 84.006 g/mol
6.4 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 Disodium succinate
C4H4O4.2Na 162.052 g/mol
5 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
10 Yeast extract 5 G_PER_L
11 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.3 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.3 G_PER_L CHEBI:76209
sodium sulfide nonahydrate

Preparation

  1. Dissolve ingredients (except bicarbonate, succinate, yeast extract, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 100% CO2 gas atmosphere. Add the bicarbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add succinate, yeast extract, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.7 - 6.8, if necessary.
  2. Note: Supplementing the medium with 1.50 g/l agar stimulates growth of strains after resuscitation from ampoules.
  3. Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.877457Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 666
2026-03-13T06:19:07.342078Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001805
2026-03-17T06:30:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.639089+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SUCCINICLASTICUM MEDIUM" to "succiniclasticum_medium" for consistent matching
2026-05-20T06:02:06.263932+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.7-6.8' -> {'min': 6.7, 'max': 6.8}
2026-06-05T07:41:47.220237+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.924597+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.