acidiferrobacter_ms_medium

CultureMech:001811 bacterial DEFINED LIQUID
Original name: ACIDIFERROBACTER (MS) MEDIUM

Overview

External ontology
mediadive.medium:670 (source link unavailable) — ACIDIFERROBACTER (MS) MEDIUM
Applications
  • Microbial cultivation
pH
2.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium670.pdf Stock follow-up: quantities distinguish base-water inputs, stock strengths and batch doses; no measured final volume or new growth claim is inferred. Existing chemical mappings and variant links are retained, not independently validated. Note: Incubate statically without shaking. This correction covers the base formulation only; strain-specific alternatives require separate review.

Composition

flowchart LR
    CultureMech_001811["acidiferrobacter_ms_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001811 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001811 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_K2HPO4["K2HPO4"]
    CultureMech_001811 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_KCl["KCl"]
    CultureMech_001811 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Distilled_water["Distilled water"]
    CultureMech_001811 --> ing4_Distilled_water
    ing5_H2SO4["H2SO4"]
    CultureMech_001811 --> ing5_H2SO4

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2 G_PER_L CHEBI:62946
ammonium sulfate
 Source batch input: 2 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration.
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Source batch input: 0.25 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration.
3 K2HPO4
HO4P.2K 174.174 g/mol
0.1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Source batch input: 0.1 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration.
4 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
 Source batch input: 0.1 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration.
5 Distilled water 0.95 L mediadive.compound:4 (source link unavailable)
Distilled water
 Distilled-water input to the base per recipe batch, not final medium volume or stock carrier volume.
6 H2SO4 Use 4 N H2SO4 to adjust the base to pH 2.2 before autoclaving; addition amount is not quantified. Normality is retained as text, not converted to an unsupported concentration unit. This is not the 0.2 N iron-stock carrier.

Preparation

  1. Dissolve ingredients except ferrous sulfate and adjust pH to 2.2 with 4 N H2SO4. Sterilize medium by autoclaving for 15 min at 121°C. Before use, add FeSO4 x 7 H2O from a 40% (w/v) stock solution prepared with 0.2 N H2SO4 and filter-sterilized under 100% N2 gas atmosphere.

Solutions

FeSO4 x 7 H2O stock

Dose: 0.05 L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 40 PERCENT_W_V Source-explicit stock strength, not a final-medium concentration. The former flattened value is replaced at the stock stage; no molecular-weight or density conversion is inferred.
H2SO4 Stock carrier is 0.2 N H2SO4; carrier amount is unspecified. Normality is retained as source text because no NORMAL unit exists in the concentration enum. This is distinct from the 4 N reagent used to adjust base pH.
50 ml stock addition per recipe batch, not per liter of an inferred final medium. Carrier amount and final-medium concentration are not inferred.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.893371Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 670
2026-03-13T06:19:07.389283Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001811
2026-03-17T06:30:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.693487+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ACIDIFERROBACTER (MS) MEDIUM" to "acidiferrobacter_ms_medium" for consistent matching
2026-06-05T07:40:47.192280+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.555678+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T12:21:30.028294+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.
2026-10-08T04:59:45.451604+00:00 codex CORRECTED_DSMZ546_659_670_STOCK_STAGES Restored source-explicit stock strengths/doses and base-water inputs; prior excerpts, history and mappings preserved. No strain-variant or growth curation is claimed.