thermococcus_profundus_medium

CultureMech:001814 archaea COMPLEX LIQUID
Original name: THERMOCOCCUS PROFUNDUS MEDIUM

Overview

External ontology
mediadive.medium:673 (source link unavailable) — THERMOCOCCUS PROFUNDUS MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium673.pdf

Composition

flowchart LR
    CultureMech_001814["thermococcus_profundus_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001814 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001814 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001814 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001814 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_001814 --> ing4_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001814 --> ing5_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing6_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_001814 --> ing6_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing6_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001814 --> ing7_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing8_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001814 --> ing8_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing8_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing9_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001814 --> ing9_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing9_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing10_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
    CultureMech_001814 --> ing10_VOSO4_x_2_H2O
    CHEBI_87009["CHEBI:87009"]
    ing10_VOSO4_x_2_H2O -.-> CHEBI_87009
    ing11_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001814 --> ing11_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing11_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing12_NaCl["NaCl"]
    CultureMech_001814 --> ing12_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing12_NaCl -.-> CHEBI_26710
    ing13_Resazurin["Resazurin"]
    CultureMech_001814 --> ing13_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing13_Resazurin -.-> CHEBI_8806
    ing14_Yeast_extract["Yeast extract"]
    CultureMech_001814 --> ing14_Yeast_extract
    ing15_Sulfur["Sulfur"]
    CultureMech_001814 --> ing15_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing15_Sulfur -.-> CHEBI_26833
    ing16_Peptone["Peptone"]
    CultureMech_001814 --> ing16_Peptone

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.3 G_PER_L CHEBI:62946
ammonium sulfate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.28 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.07 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.02 G_PER_L CHEBI:86254
iron trichloride hexahydrate
6 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.0018 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
7 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0045 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
8 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.00022 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
9 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
5e-05 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
10 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
3e-05 G_PER_L CHEBI:75213
sodium molybdate dihydrate
11 VOSO4 x 2 H2O
2H2O.O4S.OV 199.036 g/mol
3e-05 G_PER_L CHEBI:87009
vanadyl sulfate dihydrate
12 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
1e-05 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
13 NaCl
Cl.Na 58.443 g/mol
25 G_PER_L CHEBI:26710
sodium chloride
14 Resazurin
C12H7NO4 229.191 g/mol
0.001 G_PER_L CHEBI:8806
Resazurin
15 Yeast extract 1 G_PER_L
16 Sulfur
S 32.067 g/mol
5 G_PER_L CHEBI:26833
sulfur atom
17 Peptone 5 G_PER_L

Preparation

  1. Prepare the medium anaerobically under 100% nitrogen. The following constituents are prepared separately and added to the autoclaved mineral salt solution: Yeast extract (20 ml of 10% w/v solution)-boiled for a few minutes not autoclaved; sulfur (10 g)-sterilized by steaming for 3 h on each of three successive days; Na2S x 9 H2O (10 ml of 3% w/v solution)-autoclaved under nitrogen atmosphere.
  2. Adjust final pH to 7.2.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.900659Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 673
2026-03-13T06:19:07.414064Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001814
2026-03-17T06:30:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.722305+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "THERMOCOCCUS PROFUNDUS MEDIUM" to "thermococcus_profundus_medium" for consistent matching
2026-06-05T07:41:49.951357+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:12.930205+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T12:21:30.028294+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.