bacillus_thermantarcticus_medium

CultureMech:001815 bacterial COMPLEX LIQUID
Original name: BACILLUS THERMANTARCTICUS MEDIUM

Overview

External ontology
mediadive.medium:675 (source link unavailable) — BACILLUS THERMANTARCTICUS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 5.6, 'max': 5.8}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium675.pdf

Composition

flowchart LR
    CultureMech_001815["bacillus_thermantarcticus_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001815 --> ing0_Yeast_extract
    ing1_NaCl["NaCl"]
    CultureMech_001815 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_Soil_extract["Soil extract"]
    CultureMech_001815 --> ing2_Soil_extract

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Yeast extract 6 G_PER_L FOODON:03315426
Yeast extract
2 NaCl
Cl.Na 58.443 g/mol
3 G_PER_L CHEBI:26710
sodium chloride
3 Soil extract 500 G_PER_L MICRO:0000457 (source link unavailable)
Soil extract

Preparation

  1. Adjust pH between 5.6 - 5.8.
  2. Soil extract is prepared by sieving air dried garden soil through a coarse sieve and autoclaving 400 g with 960 ml of distilled water at 121°C for one hour. After the mixture is cool and settled, the supernatant is carefully decanted, filtered through paper, autoclaved in 200 ml quantities, and stored at room temperature until cleared by sedimentation.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.904902Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 675
2026-03-13T06:19:07.418834Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001815
2026-03-17T06:30:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.728073+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BACILLUS THERMANTARCTICUS MEDIUM" to "bacillus_thermantarcticus_medium" for consistent matching
2026-05-20T06:01:09.487708+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '5.6-5.8' -> {'min': 5.6, 'max': 5.8}
2026-06-05T07:40:50.344101+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-13T00:00:00-07:00 repair_bacterial_score10_exact_terms_batch2.py GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH2 Applied local mappings for Soil extract, Yeast extract.
2026-10-07T12:21:30.028294+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.