medium_e_2

CultureMech:001831 bacterial DEFINED LIQUID
Original name: MEDIUM E-2

Overview

External ontology
mediadive.medium:694 (source link unavailable) — MEDIUM E-2
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium694.pdf

Composition

flowchart LR
    CultureMech_001831["medium_e_2"]
    ing0_NaNH4HPO4_x_4_H2O["NaNH4HPO4 x 4 H2O"]
    CultureMech_001831 --> ing0_NaNH4HPO4_x_4_H2O
    ing1_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_001831 --> ing1_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001831 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001831 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Thiamine["Thiamine"]
    CultureMech_001831 --> ing4_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing4_Thiamine -.-> CHEBI_18385
    ing5_Tap_water["Tap water"]
    CultureMech_001831 --> ing5_Tap_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Tap_water -.-> CHEBI_15377

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 NaNH4HPO4 x 4 H2O 3.5 G_PER_L
2 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
7.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 KH2PO4
H2O4P.K 136.084 g/mol
3.7 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.246476 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 Thiamine
C12H17N4OS 265.362 g/mol
0.01 G_PER_L CHEBI:18385
thiamine(1+)
6 Tap water
H2O 18.015 g/mol
1000 G_PER_L CHEBI:15377
water

Preparation

  1. Adjust pH to 7.0. n-Octane is evaporated in a desiccator as sole source of carbon.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.951509Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 694
2026-03-13T06:19:07.576533Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001831
2026-03-17T06:30:07.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.904226+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM E-2" to "medium_e_2" for consistent matching
2026-06-05T07:41:28.794019+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:05.953376+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T12:21:30.028294+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.