Overview
- External ontology
- mediadive.medium:696 (source link unavailable) — STREPTOCOCCUS SUIS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium696.pdf
Composition
flowchart LR
CultureMech_001833["streptococcus_suis_medium"]
ing0_Peptone["Peptone"]
CultureMech_001833 --> ing0_Peptone
ing1_Meat_extract["Meat extract"]
CultureMech_001833 --> ing1_Meat_extract
ing2_Yeast_extract["Yeast extract"]
CultureMech_001833 --> ing2_Yeast_extract
ing3_KH2PO4["KH2PO4"]
CultureMech_001833 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_K2HPO4["K2HPO4"]
CultureMech_001833 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001833 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
CultureMech_001833 --> ing6_MnSO4_x_4_H2O
CHEBI_86358["CHEBI:86358"]
ing6_MnSO4_x_4_H2O -.-> CHEBI_86358
ing7_Glucose["Glucose"]
CultureMech_001833 --> ing7_Glucose
CHEBI_17234["CHEBI:17234"]
ing7_Glucose -.-> CHEBI_17234
ing8_Lactose["Lactose"]
CultureMech_001833 --> ing8_Lactose
CHEBI_17716["CHEBI:17716"]
ing8_Lactose -.-> CHEBI_17716
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001833 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_Bovine_serum["Bovine serum"]
CultureMech_001833 --> ing10_Bovine_serum
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 10 G_PER_L | ||
| 2 | Meat extract | 8 G_PER_L | ||
| 3 | Yeast extract | 3 G_PER_L | ||
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
|
0.05 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
|
| 8 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 9 |
Lactose
C12H22O11 342.297 g/mol
|
5 G_PER_L |
CHEBI:17716
lactose
|
|
| 10 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 11 | Bovine serum | 50 G_PER_L |
Preparation
- pH 6.8
- After sterilization add 5% bovine serum.
- For anaerobic handling prepare and distribute into tubes under 100% N2 by using the Hungate technique.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.954194Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 696 |
| 2026-03-13T06:19:07.585233Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001833 |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.913507+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "STREPTOCOCCUS SUIS MEDIUM" to "streptococcus_suis_medium" for consistent matching |
| 2026-06-05T07:41:47.060921+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.861895+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T12:21:30.028294+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |