Overview
- External ontology
- mediadive.medium:700 (source link unavailable) — ALKALIPHILIC SPIROCHAETE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.7
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium700.pdf
Composition
flowchart LR
CultureMech_001839["alkaliphilic_spirochaete_medium"]
ing0_Na2CO3["Na2CO3"]
CultureMech_001839 --> ing0_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing0_Na2CO3 -.-> CHEBI_29377
ing1_NaHCO3["NaHCO3"]
CultureMech_001839 --> ing1_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing1_NaHCO3 -.-> CHEBI_32139
ing2_NaCl["NaCl"]
CultureMech_001839 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_001839 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_NH4Cl["NH4Cl"]
CultureMech_001839 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_KCl["KCl"]
CultureMech_001839 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001839 --> ing6_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_x_9_H2O -.-> CHEBI_76209
ing7_Yeast_extract["Yeast extract"]
CultureMech_001839 --> ing7_Yeast_extract
ing8_Sucrose["Sucrose"]
CultureMech_001839 --> ing8_Sucrose
CHEBI_17992["CHEBI:17992"]
ing8_Sucrose -.-> CHEBI_17992
ing9_Biotin["Biotin"]
CultureMech_001839 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Folic_acid["Folic acid"]
CultureMech_001839 --> ing10_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing10_Folic_acid -.-> CHEBI_27470
ing11_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001839 --> ing11_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing11_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing12_Thiamine_HCl["Thiamine HCl"]
CultureMech_001839 --> ing12_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing12_Thiamine_HCl -.-> CHEBI_49105
ing13_Riboflavin["Riboflavin"]
CultureMech_001839 --> ing13_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing13_Riboflavin -.-> CHEBI_17015
ing14_Nicotinic_acid["Nicotinic acid"]
CultureMech_001839 --> ing14_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing14_Nicotinic_acid -.-> CHEBI_15940
ing15_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001839 --> ing15_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing15_Calcium_D_____pantothenate -.-> CHEBI_31345
ing16_Vitamin_B12["Vitamin B12"]
CultureMech_001839 --> ing16_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing16_Vitamin_B12 -.-> CHEBI_176843
ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001839 --> ing17_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
ing18__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_001839 --> ing18__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing18__DL__alpha_Lipoic_acid -.-> CHEBI_16494
ing19_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001839 --> ing19_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing19_Nitrilotriacetic_acid -.-> CHEBI_44557
ing20_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001839 --> ing20_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing20_MgSO4_x_7_H2O -.-> CHEBI_31795
ing21_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001839 --> ing21_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing21_MnSO4_x_H2O -.-> CHEBI_86364
ing22_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001839 --> ing22_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing22_FeSO4_x_7_H2O -.-> CHEBI_75836
ing23_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001839 --> ing23_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing23_CoSO4_x_7_H2O -.-> CHEBI_91244
ing24_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001839 --> ing24_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing24_CaCl2_x_2_H2O -.-> CHEBI_86158
ing25_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001839 --> ing25_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing25_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing26_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001839 --> ing26_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing26_CuSO4_x_5_H2O -.-> CHEBI_31440
ing27_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001839 --> ing27_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing27_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing28_H3BO3["H3BO3"]
CultureMech_001839 --> ing28_H3BO3
CHEBI_33118["CHEBI:33118"]
ing28_H3BO3 -.-> CHEBI_33118
ing29_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001839 --> ing29_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing29_Na2MoO4_x_2_H2O -.-> CHEBI_75213
CultureMech_001839 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2CO3
CO3.2Na 105.988 g/mol
|
10 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 2 |
NaHCO3
CHO3.Na 84.006 g/mol
|
15 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
11.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 10.0, 1.0] |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
1 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 8 | Yeast extract | 0.5 G_PER_L | ||
| 9 |
Sucrose
C12H22O11 342.297 g/mol
|
5 G_PER_L |
CHEBI:17992
sucrose
|
|
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 11 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 12 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 13 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 14 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 15 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 16 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 17 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 18 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 19 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 20 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 21 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 22 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 23 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 24 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 25 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 26 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 27 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 28 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 29 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 30 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 31 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 32 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Preparation
- Prepare the medium under N2, omitting Na2CO3, Na HCO3, Na2S, sucrose and vitamin solution.
- Boil the medium and cool under nitrogen, add the Na2CO3 and NaHCO3 and adjust the pH to 9.7 with 6N NaOH (about 15 ml). Sterilize by autoclaving and add the Na2S (neutralized), vitamins and sucrose from sterile stock solutions.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.960491Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 700 |
| 2026-02-06T05:10:51.226744Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:38.119776Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:07.621609Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001839 |
| 2026-03-17T06:30:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:19.953480+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALKALIPHILIC SPIROCHAETE MEDIUM" to "alkaliphilic_spirochaete_medium" for consistent matching |
| 2026-06-05T07:40:48.032250+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.846493+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |