haloanaerobacter_chitinovorans_medium

CultureMech:001840 bacterial COMPLEX LIQUID
Original name: HALOANAEROBACTER CHITINOVORANS MEDIUM

Overview

External ontology
mediadive.medium:701 (source link unavailable) — HALOANAEROBACTER CHITINOVORANS MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium701.pdf

Composition

flowchart LR
    CultureMech_001840["haloanaerobacter_chitinovorans_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_001840 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001840 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001840 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001840 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_KCl["KCl"]
    CultureMech_001840 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_001840 --> ing5_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing6_NaCl["NaCl"]
    CultureMech_001840 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_NaHCO3["NaHCO3"]
    CultureMech_001840 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_Na2CO3["Na2CO3"]
    CultureMech_001840 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001840 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_Yeast_extract["Yeast extract"]
    CultureMech_001840 --> ing10_Yeast_extract
    ing11_L_Cysteine["L-Cysteine"]
    CultureMech_001840 --> ing11_L_Cysteine
    CHEBI_17561["CHEBI:17561"]
    ing11_L_Cysteine -.-> CHEBI_17561
    ing12_Resazurin["Resazurin"]
    CultureMech_001840 --> ing12_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing12_Resazurin -.-> CHEBI_8806
    ing13_Substrate["Substrate"]
    CultureMech_001840 --> ing13_Substrate
    ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001840 --> ing14_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing15_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001840 --> ing15_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing15_MnSO4_x_H2O -.-> CHEBI_86364
    ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001840 --> ing16_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing17_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001840 --> ing17_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing17_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001840 --> ing18_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001840 --> ing19_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing20_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001840 --> ing20_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing20_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing21_H3BO3["H3BO3"]
    CultureMech_001840 --> ing21_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing21_H3BO3 -.-> CHEBI_33118
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001840 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001840 --> ing23_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing24_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001840 --> ing24_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing24_Na2SeO3_x_5_H2O -.-> CHEBI_131361

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
7 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
12.6 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 9.6, 3.0]
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.6 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.5, 0.1]
5 KCl
Cl.K 74.551 g/mol
3.8 G_PER_L CHEBI:32588
potassium chloride
6 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
0.4 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
7 NaCl
Cl.Na 58.443 g/mol
11.0 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 10.0, 1.0]
8 NaHCO3
CHO3.Na 84.006 g/mol
3 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 Na2CO3
CO3.2Na 105.988 g/mol
1 G_PER_L CHEBI:29377
sodium carbonate
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 Yeast extract 1 G_PER_L
12 L-Cysteine
C3H7NO2S 121.161 g/mol
0.5 G_PER_L CHEBI:17561
L-cysteine
13 Resazurin
C12H7NO4 229.191 g/mol
0.001 G_PER_L CHEBI:8806
Resazurin
14 Substrate 5 G_PER_L
15 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
16 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
17 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
18 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
19 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
20 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
21 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
22 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
24 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.025 G_PER_L CHEBI:34887
nickel dichloride
25 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate

Preparation

  1. pH 7.2
  2. Prepare the medium without the Na2S, Na2CO3, NaHCO3, L-cysteine and substrate. Boil the medium and cool under a stream of nitrogen. Add the Na2CO3 and NaHCO3 . Adjust the pH to 7.2. If chitin is used as the substrate it must be added before autoclaving. Autoclave the medium and add the Na2S (from a sterile, neutralized stock solution prepared under nitrogen), L-cysteine (from a stock solution prepared under nitrogen), and the glucose or N-acetylglucosamine (if used as substrate) from sterile stock solution prepared under nitrogen. Check the final pH.
  3. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.964837Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 701
2026-03-13T03:09:09.195380Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:07.634603Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001840
2026-03-17T06:30:15.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.967990+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOANAEROBACTER CHITINOVORANS MEDIUM" to "haloanaerobacter_chitinovorans_medium" for consistent matching
2026-06-05T07:41:07.349857+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.383247+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.