butyrivibrio_sp_medium

Overview

External ontology

mediadive.medium:704 (source link unavailable) — BUTYRIVIBRIO SP. MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 6.7, 'max': 6.8}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium704.pdf Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_001843["butyrivibrio_sp_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_001843 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_Trypticase_peptone["Trypticase peptone"]
    CultureMech_001843 --> ing1_Trypticase_peptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_001843 --> ing2_Yeast_extract
    ing3_Glycerol["Glycerol"]
    CultureMech_001843 --> ing3_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing3_Glycerol -.-> CHEBI_17754
    ing4_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001843 --> ing4_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Sodium_resazurin -.-> CHEBI_8806
    ing5_Na2CO3["Na2CO3"]
    CultureMech_001843 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_D_Glucose["D-Glucose"]
    CultureMech_001843 --> ing6_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing6_D_Glucose -.-> CHEBI_17634
    ing7_Maltose["Maltose"]
    CultureMech_001843 --> ing7_Maltose
    CHEBI_17306["CHEBI:17306"]
    ing7_Maltose -.-> CHEBI_17306
    ing8_Cellobiose["Cellobiose"]
    CultureMech_001843 --> ing8_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing8_Cellobiose -.-> CHEBI_17057
    ing9_Starch["Starch"]
    CultureMech_001843 --> ing9_Starch
    CHEBI_28017["CHEBI:28017"]
    ing9_Starch -.-> CHEBI_28017
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001843 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001843 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_KH2PO4["KH2PO4"]
    CultureMech_001843 --> ing12_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing12_KH2PO4 -.-> CHEBI_63036
    ing13_NaCl["NaCl"]
    CultureMech_001843 --> ing13_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing13_NaCl -.-> CHEBI_26710
    ing14__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001843 --> ing14__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing14__NH4_2SO4 -.-> CHEBI_62946
    ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001843 --> ing15_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing16_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001843 --> ing16_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing16_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing17_Acetic_acid["Acetic acid"]
    CultureMech_001843 --> ing17_Acetic_acid
    CHEBI_15366["CHEBI:15366"]
    ing17_Acetic_acid -.-> CHEBI_15366
    ing18_Propionic_acid["Propionic acid"]
    CultureMech_001843 --> ing18_Propionic_acid
    CHEBI_30768["CHEBI:30768"]
    ing18_Propionic_acid -.-> CHEBI_30768
    ing19_Butyric_acid["Butyric acid"]
    CultureMech_001843 --> ing19_Butyric_acid
    CHEBI_30772["CHEBI:30772"]
    ing19_Butyric_acid -.-> CHEBI_30772
    ing20_n_Valeric_acid["n-Valeric acid"]
    CultureMech_001843 --> ing20_n_Valeric_acid
    CHEBI_17418["CHEBI:17418"]
    ing20_n_Valeric_acid -.-> CHEBI_17418
    ing21_iso_Butyric_acid["iso-Butyric acid"]
    CultureMech_001843 --> ing21_iso_Butyric_acid
    CHEBI_16135["CHEBI:16135"]
    ing21_iso_Butyric_acid -.-> CHEBI_16135
    ing22_Tryptone["Tryptone"]
    CultureMech_001843 --> ing22_Tryptone
    ing23_Yeast_extract["Yeast extract"]
    CultureMech_001843 --> ing23_Yeast_extract
    ing24_Sodium_chloride["Sodium chloride"]
    CultureMech_001843 --> ing24_Sodium_chloride
    ing24_Sodium_chloride -.-> CHEBI_26710
    ing25_iso_Valeric_acid["iso-Valeric acid"]
    CultureMech_001843 --> ing25_iso_Valeric_acid
    CHEBI_28484["CHEBI:28484"]
    ing25_iso_Valeric_acid -.-> CHEBI_28484
    ing26_Haemin["Haemin"]
    CultureMech_001843 --> ing26_Haemin
    CHEBI_50385["CHEBI:50385"]
    ing26_Haemin -.-> CHEBI_50385
    ing27_NaOH["NaOH"]
    CultureMech_001843 --> ing27_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing27_NaOH -.-> CHEBI_32145

Ingredients (28)

Preparation

1. Dissolve ingredients (except carbonate, carbohydrates, cysteine and sulfide) and sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Add the carbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add glucose, maltose, cellobiose, starch, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Cellobiose should be sterilized by filtration. Adjust pH of complete medium to 6.7 - 6.8, if necessary.
2. Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C.
3. Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.

Target organisms

No target organisms are recorded.

Provenance