acidimicrobium_medium

CultureMech:001846 bacterial COMPLEX LIQUID
Original name: ACIDIMICROBIUM MEDIUM

Overview

External ontology
mediadive.medium:709 (source link unavailable) — ACIDIMICROBIUM MEDIUM
Applications
  • Microbial cultivation
pH
2.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium709.pdf

Composition

flowchart LR
    CultureMech_001846["acidimicrobium_medium"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001846 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001846 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_K2HPO4["K2HPO4"]
    CultureMech_001846 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_KCl["KCl"]
    CultureMech_001846 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001846 --> ing4_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_001846 --> ing5_Yeast_extract

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.4 G_PER_L CHEBI:62946
ammonium sulfate
3 K2HPO4
HO4P.2K 174.174 g/mol
0.2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
5 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.01 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
6 Yeast extract 0.25 G_PER_L

Preparation

  1. Dissolve ingredients except yeast extract and adjust pH to 2.0 with H2SO4. After autoclaving add yeast extract from a sterile stock solution.
  2. Note: For autotrophic growth omit yeast extract and add to the medium 13.90 g/l FeSO4 x 7 H2O. Adjust medium pH to 1.7 with H2SO4 prior to autoclaving.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.986879Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 709
2026-03-13T06:19:07.699114Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001846
2026-03-17T06:30:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:20.041293+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ACIDIMICROBIUM MEDIUM" to "acidimicrobium_medium" for consistent matching
2026-06-05T07:40:47.297114+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.594456+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T12:21:30.028294+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.