Overview
- External ontology
- mediadive.medium:717 (source link unavailable) — DESULFITOBACTERIUM (PCE) MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.2}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium717.pdf
Composition
flowchart LR
CultureMech_001855["desulfitobacterium_pce_medium"]
ing0__NH4_H2PO4["(NH4)H2PO4"]
CultureMech_001855 --> ing0__NH4_H2PO4
CHEBI_62982["CHEBI:62982"]
ing0__NH4_H2PO4 -.-> CHEBI_62982
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001855 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
CultureMech_001855 --> ing2_Ca_NO3_2_x_4_H2O
CHEBI_86159["CHEBI:86159"]
ing2_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
ing3_Yeast_extract["Yeast extract"]
CultureMech_001855 --> ing3_Yeast_extract
ing4_Sodium_resazurin["Sodium resazurin"]
CultureMech_001855 --> ing4_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Sodium_resazurin -.-> CHEBI_8806
ing5_Na2CO3["Na2CO3"]
CultureMech_001855 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_Na_L_lactate["Na-L-lactate"]
CultureMech_001855 --> ing6_Na_L_lactate
CHEBI_232798["CHEBI:232798"]
ing6_Na_L_lactate -.-> CHEBI_232798
ing7_Na2_fumarate["Na2-fumarate"]
CultureMech_001855 --> ing7_Na2_fumarate
CHEBI_115156["CHEBI:115156"]
ing7_Na2_fumarate -.-> CHEBI_115156
ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001855 --> ing8_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_x_9_H2O -.-> CHEBI_76209
ing9_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001855 --> ing9_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing9_Nitrilotriacetic_acid -.-> CHEBI_44557
ing10_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001855 --> ing10_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing10_MnSO4_x_H2O -.-> CHEBI_86364
ing11_NaCl["NaCl"]
CultureMech_001855 --> ing11_NaCl
CHEBI_26710["CHEBI:26710"]
ing11_NaCl -.-> CHEBI_26710
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001855 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001855 --> ing13_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing13_CoSO4_x_7_H2O -.-> CHEBI_91244
ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001855 --> ing14_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001855 --> ing15_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001855 --> ing16_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing16_CuSO4_x_5_H2O -.-> CHEBI_31440
ing17_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001855 --> ing17_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing17_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing18_H3BO3["H3BO3"]
CultureMech_001855 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001855 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001855 --> ing20_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001855 --> ing21_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001855 --> ing22_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing23_Biotin["Biotin"]
CultureMech_001855 --> ing23_Biotin
CHEBI_15956["CHEBI:15956"]
ing23_Biotin -.-> CHEBI_15956
ing24_Folic_acid["Folic acid"]
CultureMech_001855 --> ing24_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing24_Folic_acid -.-> CHEBI_27470
ing25_Thiamine_HCl["Thiamine HCl"]
CultureMech_001855 --> ing25_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing25_Thiamine_HCl -.-> CHEBI_49105
ing26_Riboflavin["Riboflavin"]
CultureMech_001855 --> ing26_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing26_Riboflavin -.-> CHEBI_17015
ing27_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001855 --> ing27_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing27_Calcium_D_____pantothenate -.-> CHEBI_31345
ing28__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_001855 --> ing28__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing28__DL__alpha_Lipoic_acid -.-> CHEBI_16494
ing29_D_____biotin["D-(+)-biotin"]
CultureMech_001855 --> ing29_D_____biotin
ing29_D_____biotin -.-> CHEBI_15956
CultureMech_001855 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)H2PO4
H2O4P.H4N 115.025 g/mol
|
2.84585 G_PER_L |
CHEBI:62982
ammonium dihydrogen phosphate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.0988142 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.0988142, 3.0] |
| 3 |
Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.0494071 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
|
| 4 | Yeast extract | 0.0988142 G_PER_L | ||
| 5 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000494071 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1.48221 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 7 |
Na-L-lactate
C3H5O3.Na 112.06 g/mol
|
4.94071 G_PER_L |
CHEBI:232798
sodium L-lactate
|
|
| 8 |
Na2-fumarate
C4H2O4.2Na 160.036 g/mol
|
3.16206 G_PER_L |
CHEBI:115156
disodium fumarate
|
|
| 9 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.0988142 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 10 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 11 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 12 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 15 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 16 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 17 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 18 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 21 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 22 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 23 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 24 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 25 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 26 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 27 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 28 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 29 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 30 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 31 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Preparation
- Dissolve ingredients except carbonate, lactate, fumarate, vitamins and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add lactate, fumarate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Stock solutions of fumarate and vitamins should be sterilized by filtration. The pH of the complete medium should be at 7.0 - 7.2.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solutions
Seven vitamins solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Thiamine-HCl x 2 H2O | 0.2 G_PER_L | |
| Nicotinic acid | 0.2 G_PER_L | [Merged 2 duplicates: 0.05, 0.2] |
| p-Aminobenzoic acid | 0.08 G_PER_L | [Merged 2 duplicates: 0.05, 0.08] |
| Pyridoxine hydrochloride | 0.3 G_PER_L | [Merged 2 duplicates: 0.1, 0.3] |
| Vitamin B12 | 0.1 G_PER_L | [Merged 2 duplicates: 0.001, 0.1] |
Wolin's vitamin solution (10x)
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Nicotinic acid | 0.05 G_PER_L | [Merged 2 duplicates: 0.05, 0.2] |
| p-Aminobenzoic acid | 0.05 G_PER_L | [Merged 2 duplicates: 0.05, 0.08] |
| Pyridoxine hydrochloride | 0.1 G_PER_L | [Merged 2 duplicates: 0.1, 0.3] |
| Vitamin B12 | 0.001 G_PER_L | [Merged 2 duplicates: 0.001, 0.1] |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.016456Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 717 |
| 2026-01-28T19:48:37.428464Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-06T05:10:48.640760Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:34.302867Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:19:07.787445Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001855 |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:20.140652+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFITOBACTERIUM (PCE) MEDIUM" to "desulfitobacterium_pce_medium" for consistent matching |
| 2026-05-20T06:01:13.398419+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2} |
| 2026-06-05T07:40:56.035100+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.351585+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-07T23:42:25.364898+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 1 -> 'Seven vitamins solution' @ 1 ml/l; 0 -> "Wolin's vitamin solution (10x)" @ 1 ml/l. 4 component(s) were SUMMED across stocks by the flattening and are restored to each stock's own value, verified by exact sum: nicotinic acid, p-aminobenzoic acid, pyridoxine hydrochloride, vitamin b12. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |