Overview
- External ontology
- mediadive.medium:719 (source link unavailable) — ANAEROFILUM MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.8, 'max': 7.0}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium719.pdf Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_001857["anaerofilum_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001857 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001857 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_001857 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_NH4Cl["NH4Cl"]
CultureMech_001857 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001857 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Yeast_extract["Yeast extract"]
CultureMech_001857 --> ing5_Yeast_extract
ing6_Na_acetate["Na-acetate"]
CultureMech_001857 --> ing6_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing6_Na_acetate -.-> CHEBI_32954
ing7_Na_formate["Na-formate"]
CultureMech_001857 --> ing7_Na_formate
CHEBI_62965["CHEBI:62965"]
ing7_Na_formate -.-> CHEBI_62965
ing8_Sodium_resazurin["Sodium resazurin"]
CultureMech_001857 --> ing8_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Sodium_resazurin -.-> CHEBI_8806
ing9_NaHCO3["NaHCO3"]
CultureMech_001857 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_D_Glucose["D-Glucose"]
CultureMech_001857 --> ing10_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing10_D_Glucose -.-> CHEBI_17634
ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001857 --> ing11_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001857 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_HCl["HCl"]
CultureMech_001857 --> ing13_HCl
CHEBI_17883["CHEBI:17883"]
ing13_HCl -.-> CHEBI_17883
ing14_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001857 --> ing14_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing14_FeCl2_x_4_H2O -.-> CHEBI_86249
ing15_ZnCl2["ZnCl2"]
CultureMech_001857 --> ing15_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing15_ZnCl2 -.-> CHEBI_49976
ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001857 --> ing16_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
ing17_H3BO3["H3BO3"]
CultureMech_001857 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001857 --> ing18_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001857 --> ing19_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001857 --> ing20_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001857 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_Isobutyric_acid["Isobutyric acid"]
CultureMech_001857 --> ing22_Isobutyric_acid
CHEBI_16135["CHEBI:16135"]
ing22_Isobutyric_acid -.-> CHEBI_16135
ing23_Tryptone["Tryptone"]
CultureMech_001857 --> ing23_Tryptone
ing24_Yeast_extract["Yeast extract"]
CultureMech_001857 --> ing24_Yeast_extract
ing25_Sodium_chloride["Sodium chloride"]
CultureMech_001857 --> ing25_Sodium_chloride
ing25_Sodium_chloride -.-> CHEBI_26710
ing26_Valeric_acid["Valeric acid"]
CultureMech_001857 --> ing26_Valeric_acid
CHEBI_17418["CHEBI:17418"]
ing26_Valeric_acid -.-> CHEBI_17418
ing27_Isovaleric_acid["Isovaleric acid"]
CultureMech_001857 --> ing27_Isovaleric_acid
CHEBI_28484["CHEBI:28484"]
ing27_Isovaleric_acid -.-> CHEBI_28484
ing28_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001857 --> ing28_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing28_FeSO4_x_7_H2O -.-> CHEBI_75836
ing29_H2SO4["H2SO4"]
CultureMech_001857 --> ing29_H2SO4
CHEBI_26836["CHEBI:26836"]
ing29_H2SO4 -.-> CHEBI_26836
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.488759 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.391007 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.391007 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.391007 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0488759 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 | Yeast extract | 0.977517 G_PER_L | ||
| 7 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.977517 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 8 |
Na-formate
CHO2.Na 68.007 g/mol
|
1.95503 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 9 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000488759 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
3.91007 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 11 |
D-Glucose
C6H12O6 180.156 g/mol
|
4.88759 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 12 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.488759 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.488759 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 14 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 15 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 16 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 17 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 20 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 21 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 |
Isobutyric acid
C4H8O2 88.106 g/mol
|
23 G_PER_L |
CHEBI:16135
isobutyric acid
|
|
| 24 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 25 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 26 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 27 |
Valeric acid
C5H10O2 102.133 g/mol
|
27 G_PER_L |
CHEBI:17418
valeric acid
|
|
| 28 |
Isovaleric acid
C5H10O2 102.133 g/mol
|
27 G_PER_L |
CHEBI:28484
isovaleric acid
|
|
| 29 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 30 |
H2SO4
H2O4S 98.079 g/mol
|
1000 G_PER_L |
CHEBI:26836
sulfuric acid
|
Preparation
- Dissolve ingredients except bicarbonate, glucose, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 6.8 - 7.0, if necessary.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
- Adjust pH to 7.5 with concentrated NaOH.
- The ferrous sulfate solution is not stable and should be freshly prepared.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.031883Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 719 |
| 2026-03-13T06:19:07.817933Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001857 |
| 2026-03-17T06:30:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:20.178343+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ANAEROFILUM MEDIUM" to "anaerofilum_medium" for consistent matching |
| 2026-05-20T06:01:08.666519+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0} |
| 2026-06-05T07:40:49.070617+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.281349+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |