acidithiobacillus_thiooxidans_medium

CultureMech:001858 bacterial DEFINED LIQUID
Original name: ACIDITHIOBACILLUS THIOOXIDANS MEDIUM

Overview

External ontology
mediadive.medium:71 (source link unavailable) — ACIDITHIOBACILLUS THIOOXIDANS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 4.4, 'max': 4.7}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium71.pdf

Composition

flowchart LR
    CultureMech_001858["acidithiobacillus_thiooxidans_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001858 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001858 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001858 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001858 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_001858 --> ing4_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing4_Na2S2O3_x_5_H2O -.-> CHEBI_32150

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
3 G_PER_L CHEBI:62946
ammonium sulfate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
5 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate

Preparation

  1. Prepare the medium without thiosulfate, adjust pH to 4.4 - 4.7 and autoclave at 121°C for 15 min. Sterilize thiosulfate separately by filtration and add after autoclaving.
  2. Note: Incubate statically without shaking.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.088251Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 71
2026-03-13T06:19:07.824881Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001858
2026-03-17T06:30:10.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:20.186982+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ACIDITHIOBACILLUS THIOOXIDANS MEDIUM" to "acidithiobacillus_thiooxidans_medium" for consistent matching
2026-05-20T06:01:07.502656+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '4.4-4.7' -> {'min': 4.4, 'max': 4.7}
2026-06-05T07:40:47.364389+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.621227+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-13T00:00:00-07:00 repair_komodo_71_acidithiobacillus_score10.py RESOLVED_KOMODO_71_ACIDITHIOBACILLUS_TOPOLOGY Moved exact KOMODO Medium 71 and its strain wrapper under DSMZ Medium 71.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.