Overview
- External ontology
- mediadive.medium:727 (source link unavailable) — CLOSTRIDIUM ULTUNENSE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium727.pdf
Composition
flowchart LR
CultureMech_001862["clostridium_ultunense_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001862 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_Na2HPO4["Na2HPO4"]
CultureMech_001862 --> ing1_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing1_Na2HPO4 -.-> CHEBI_34683
ing2_NH4Cl["NH4Cl"]
CultureMech_001862 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaCl["NaCl"]
CultureMech_001862 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001862 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001862 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_Yeast_extract["Yeast extract"]
CultureMech_001862 --> ing6_Yeast_extract
ing7_Peptone["Peptone"]
CultureMech_001862 --> ing7_Peptone
ing8_Lab_Lemco_powder["Lab Lemco powder"]
CultureMech_001862 --> ing8_Lab_Lemco_powder
ing9_Starch["Starch"]
CultureMech_001862 --> ing9_Starch
CHEBI_28017["CHEBI:28017"]
ing9_Starch -.-> CHEBI_28017
ing10_Na_acetate["Na-acetate"]
CultureMech_001862 --> ing10_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing10_Na_acetate -.-> CHEBI_32954
ing11_Resazurin["Resazurin"]
CultureMech_001862 --> ing11_Resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Resazurin -.-> CHEBI_8806
ing12_Na2CO3["Na2CO3"]
CultureMech_001862 --> ing12_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing12_Na2CO3 -.-> CHEBI_29377
ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001862 --> ing13_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing13_Na2S_x_9_H2O -.-> CHEBI_76209
ing14_Na2_EDTA["Na2-EDTA"]
CultureMech_001862 --> ing14_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing14_Na2_EDTA -.-> CHEBI_64734
ing15_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001862 --> ing15_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing15_FeCl2_x_4_H2O -.-> CHEBI_86249
ing16_ZnCl2["ZnCl2"]
CultureMech_001862 --> ing16_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing16_ZnCl2 -.-> CHEBI_49976
ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001862 --> ing17_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
ing18_H3BO3["H3BO3"]
CultureMech_001862 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001862 --> ing19_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001862 --> ing20_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
ing21_AlCl3["AlCl3"]
CultureMech_001862 --> ing21_AlCl3
CHEBI_30114["CHEBI:30114"]
ing21_AlCl3 -.-> CHEBI_30114
ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001862 --> ing22_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001862 --> ing23_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing24_NaOH["NaOH"]
CultureMech_001862 --> ing24_NaOH
CHEBI_32145["CHEBI:32145"]
ing24_NaOH -.-> CHEBI_32145
ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001862 --> ing25_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing26_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001862 --> ing26_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing26_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing27_Biotin["Biotin"]
CultureMech_001862 --> ing27_Biotin
CHEBI_15956["CHEBI:15956"]
ing27_Biotin -.-> CHEBI_15956
ing28_Folic_acid["Folic acid"]
CultureMech_001862 --> ing28_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing28_Folic_acid -.-> CHEBI_27470
ing29_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001862 --> ing29_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing29_Pyridoxine_hydrochloride -.-> CHEBI_30961
CultureMech_001862 --> more["...7 more"]
Ingredients (37)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.398804 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.428714 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.299103 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.299103 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0997009 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.0997009 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 | Yeast extract | 9.97009 G_PER_L | ||
| 8 | Peptone | 9.97009 G_PER_L | ||
| 9 | Lab Lemco powder | 4.98504 G_PER_L | ||
| 10 | Starch | 0.398804 G_PER_L |
CHEBI:28017
starch
|
|
| 11 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.398804 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 12 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000498504 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 13 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1.49551 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 14 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.299103 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 15 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 16 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
2 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 17 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 18 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 21 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 22 |
AlCl3
AlCl3 133.341 g/mol
|
0.01 G_PER_L |
CHEBI:30114
aluminium trichloride
|
|
| 23 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 24 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 25 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 26 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 27 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 28 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 29 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 30 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 31 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 32 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 33 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 34 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 35 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 36 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 37 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients except carbonate, vitamins and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of vitamins should be sterilized by filtration. Adjust pH of complete medium to 7.0, if necessay.
- First dissolve EDTA in distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add remaining compounds.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.046164Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 727 |
| 2026-02-06T05:10:28.192692Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:07.868759Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001862 |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:20.239664+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CLOSTRIDIUM ULTUNENSE MEDIUM" to "clostridium_ultunense_medium" for consistent matching |
| 2026-06-05T07:40:54.453584+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.893799+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |