sulfitobacter_pontiacus_medium

CultureMech:001869 bacterial COMPLEX LIQUID
Original name: SULFITOBACTER PONTIACUS MEDIUM

Overview

External ontology
mediadive.medium:733 (source link unavailable) — SULFITOBACTER PONTIACUS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.5, 'max': 7.8}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium733.pdf

Composition

flowchart LR
    CultureMech_001869["sulfitobacter_pontiacus_medium"]
    ing0_Na_acetate["Na-acetate"]
    CultureMech_001869 --> ing0_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing0_Na_acetate -.-> CHEBI_32954
    ing1_HEPES["HEPES"]
    CultureMech_001869 --> ing1_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing1_HEPES -.-> CHEBI_46756
    ing2_K2HPO4["K2HPO4"]
    CultureMech_001869 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_NH4Cl["NH4Cl"]
    CultureMech_001869 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_001869 --> ing4_Yeast_extract
    ing5_Peptone["Peptone"]
    CultureMech_001869 --> ing5_Peptone
    ing6_NaCl["NaCl"]
    CultureMech_001869 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001869 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001869 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_Biotin["Biotin"]
    CultureMech_001869 --> ing9_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_Biotin -.-> CHEBI_15956
    ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001869 --> ing10_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing11_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001869 --> ing11_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing11_MnSO4_x_H2O -.-> CHEBI_86364
    ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001869 --> ing12_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing13_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001869 --> ing13_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing13_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001869 --> ing14_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001869 --> ing15_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing16_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001869 --> ing16_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing16_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing17_H3BO3["H3BO3"]
    CultureMech_001869 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001869 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001869 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001869 --> ing20_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001869 --> ing21_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 Na-acetate
C2H3O2.Na 82.034 g/mol
1.64068 G_PER_L CHEBI:32954
sodium acetate
2 HEPES 7.99201 G_PER_L CHEBI:46756
HEPES
3 K2HPO4
HO4P.2K 174.174 g/mol
0.999001 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 NH4Cl
Cl.H4N 53.492 g/mol
0.499501 G_PER_L CHEBI:31206
ammonium chloride
5 Yeast extract 0.999001 G_PER_L
6 Peptone 0.499501 G_PER_L
7 NaCl
Cl.Na 58.443 g/mol
15.985 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 14.985, 1.0]
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.999001 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 0.999001, 3.0]
9 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.14995 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.04995, 0.1]
10 Biotin
C10H16N2O3S 244.316 g/mol
0.0999001 G_PER_L CHEBI:15956
biotin
11 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
12 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
13 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
14 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
15 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
16 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
17 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
18 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
21 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
22 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Prepare the HEPES, NaCl, K2HPO4 and NH4Cl as a basal salt solution, adjusting the pH to 7.5-7.8 with NaOH before sterilisation. All other components are added from sterile stock solutions.
  2. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.077447Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 733
2026-03-13T03:09:15.342961Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:07.964424Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001869
2026-03-17T06:30:23.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:20.347973+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SULFITOBACTER PONTIACUS MEDIUM" to "sulfitobacter_pontiacus_medium" for consistent matching
2026-05-20T06:02:06.362312+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.5-7.8' -> {'min': 7.5, 'max': 7.8}
2026-06-05T07:41:47.367201+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.983174+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.