Overview
- External ontology
- mediadive.medium:736 (source link unavailable) — RICH MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium736.pdf
Composition
flowchart LR
CultureMech_001872["rich_medium"]
ing0_Bacto_peptone["Bacto peptone"]
CultureMech_001872 --> ing0_Bacto_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_001872 --> ing1_Yeast_extract
ing2_Casamino_acids["Casamino acids"]
CultureMech_001872 --> ing2_Casamino_acids
ing3_Meat_extract["Meat extract"]
CultureMech_001872 --> ing3_Meat_extract
ing4_Malt_extract["Malt extract"]
CultureMech_001872 --> ing4_Malt_extract
ing5_Glycerol["Glycerol"]
CultureMech_001872 --> ing5_Glycerol
CHEBI_17754["CHEBI:17754"]
ing5_Glycerol -.-> CHEBI_17754
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001872 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_Tween_80["Tween 80"]
CultureMech_001872 --> ing7_Tween_80
CHEBI_53426["CHEBI:53426"]
ing7_Tween_80 -.-> CHEBI_53426
ing8_Agar["Agar"]
CultureMech_001872 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone | 10 G_PER_L |
MICRO:0000178 (source link unavailable)
Bacto peptone
|
|
| 2 | Yeast extract | 5 G_PER_L |
FOODON:03315426
Yeast extract
|
|
| 3 | Casamino acids | 5 G_PER_L |
FOODON:03315719
Casamino acids
|
|
| 4 | Meat extract | 2 G_PER_L | ||
| 5 | Malt extract | 5 G_PER_L |
FOODON:03301056
Malt extract
|
|
| 6 |
Glycerol
C3H8O3 92.094 g/mol
|
2 G_PER_L |
CHEBI:17754
glycerol
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
0.05 G_PER_L |
CHEBI:53426
polysorbate 80
|
|
| 9 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Final pH is 7.2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.091225Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 736 |
| 2026-01-28T19:48:41.517158Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:08.000882Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001872 |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:20.390912+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RICH MEDIUM" to "rich_medium" for consistent matching |
| 2026-06-05T07:41:44.550588+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:50.215416+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-09-13T00:00:00-07:00 | repair_bacterial_score10_exact_terms_batch7.py | GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH7 | Applied local mappings for Bacto peptone, Casamino acids, Malt extract, Yeast extract. |
| 2026-10-07T12:21:30.028294+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |