Overview
- External ontology
- mediadive.medium:738 (source link unavailable) — BPHD Medium
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium738.pdf
Composition
flowchart LR
CultureMech_001874["bphd_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_001874 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_001874 --> ing1_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing1_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing2_Na2HPO4_x_H2O["Na2HPO4 x H2O"]
CultureMech_001874 --> ing2_Na2HPO4_x_H2O
CHEBI_34683["CHEBI:34683"]
ing2_Na2HPO4_x_H2O -.-> CHEBI_34683
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001874 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001874 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_KCl["KCl"]
CultureMech_001874 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001874 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_Cresol["Cresol"]
CultureMech_001874 --> ing7_Cresol
ing8_Biotin["Biotin"]
CultureMech_001874 --> ing8_Biotin
CHEBI_15956["CHEBI:15956"]
ing8_Biotin -.-> CHEBI_15956
ing9_Folic_acid["Folic acid"]
CultureMech_001874 --> ing9_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing9_Folic_acid -.-> CHEBI_27470
ing10_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001874 --> ing10_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing10_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing11_Thiamine_HCl["Thiamine HCl"]
CultureMech_001874 --> ing11_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing11_Thiamine_HCl -.-> CHEBI_49105
ing12_Riboflavin["Riboflavin"]
CultureMech_001874 --> ing12_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing12_Riboflavin -.-> CHEBI_17015
ing13_Nicotinic_acid["Nicotinic acid"]
CultureMech_001874 --> ing13_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing13_Nicotinic_acid -.-> CHEBI_15940
ing14_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001874 --> ing14_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing14_Calcium_D_____pantothenate -.-> CHEBI_31345
ing15_Vitamin_B12["Vitamin B12"]
CultureMech_001874 --> ing15_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing15_Vitamin_B12 -.-> CHEBI_176843
ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001874 --> ing16_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
ing17__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_001874 --> ing17__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing17__DL__alpha_Lipoic_acid -.-> CHEBI_16494
ing18_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001874 --> ing18_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing18_Nitrilotriacetic_acid -.-> CHEBI_44557
ing19_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001874 --> ing19_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing19_MgSO4_x_7_H2O -.-> CHEBI_31795
ing20_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001874 --> ing20_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing20_MnSO4_x_H2O -.-> CHEBI_86364
ing21_NaCl["NaCl"]
CultureMech_001874 --> ing21_NaCl
CHEBI_26710["CHEBI:26710"]
ing21_NaCl -.-> CHEBI_26710
ing22_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001874 --> ing22_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing22_CoSO4_x_7_H2O -.-> CHEBI_91244
ing23_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001874 --> ing23_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing23_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing24_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001874 --> ing24_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing24_CuSO4_x_5_H2O -.-> CHEBI_31440
ing25_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001874 --> ing25_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing25_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing26_H3BO3["H3BO3"]
CultureMech_001874 --> ing26_H3BO3
CHEBI_33118["CHEBI:33118"]
ing26_H3BO3 -.-> CHEBI_33118
ing27_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001874 --> ing27_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing27_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing28_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001874 --> ing28_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing28_NiCl2_x_6_H2O -.-> CHEBI_34887
ing29_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001874 --> ing29_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing29_Na2SeO3_x_5_H2O -.-> CHEBI_131361
CultureMech_001874 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.42 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 3 |
Na2HPO4 x H2O
HO4P.2Na 141.958 g/mol
|
0.18 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Collapsed 2 identical duplicates: 0.1, 0.1] |
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.14 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.04, 0.1] |
| 8 | Cresol | 1 MILLIMOLAR | ||
| 9 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 10 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 11 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 12 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 13 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 14 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 15 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 16 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 17 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 18 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 19 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 20 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 21 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 22 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 23 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 24 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 25 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 26 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 27 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 28 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 29 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 30 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 31 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve phosphates separately, Adjust pH to 6.5. Add cresol/phenol and vitamin solution after autoclaving.
- (e.g.: 20 ml/l of a 4.7 g/l phenol-solution)
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.095425Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 738 |
| 2026-02-06T05:10:55.402812Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:44.281255Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:08.018303Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001874 |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:20.410280+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BPHD Medium" to "bphd_medium" for consistent matching |
| 2026-06-05T07:40:51.328172+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.954392+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:58.148462+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |