Overview
- External ontology
- mediadive.medium:739 (source link unavailable) — DESULFONEMA ISHIMOTOI MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium739.pdf
Composition
flowchart LR
CultureMech_001875["desulfonema_ishimotoi_medium"]
ing0_NaCl["NaCl"]
CultureMech_001875 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001875 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001875 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001875 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KCl["KCl"]
CultureMech_001875 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_KH2PO4["KH2PO4"]
CultureMech_001875 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_NH4Cl["NH4Cl"]
CultureMech_001875 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_001875 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_Na_acetate_x_3_H2O["Na-acetate x 3 H2O"]
CultureMech_001875 --> ing8_Na_acetate_x_3_H2O
CHEBI_32138["CHEBI:32138"]
ing8_Na_acetate_x_3_H2O -.-> CHEBI_32138
ing9_Disodium_succinate["Disodium succinate"]
CultureMech_001875 --> ing9_Disodium_succinate
CHEBI_63675["CHEBI:63675"]
ing9_Disodium_succinate -.-> CHEBI_63675
ing10_Isobutyric_acid["Isobutyric acid"]
CultureMech_001875 --> ing10_Isobutyric_acid
CHEBI_16135["CHEBI:16135"]
ing10_Isobutyric_acid -.-> CHEBI_16135
ing11_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001875 --> ing11_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing11_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing12_Na2CO3["Na2CO3"]
CultureMech_001875 --> ing12_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing12_Na2CO3 -.-> CHEBI_29377
ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001875 --> ing13_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing13_Na2S_x_9_H2O -.-> CHEBI_76209
ing14_HCl["HCl"]
CultureMech_001875 --> ing14_HCl
CHEBI_17883["CHEBI:17883"]
ing14_HCl -.-> CHEBI_17883
ing15_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001875 --> ing15_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing15_FeCl2_x_4_H2O -.-> CHEBI_86249
ing16_ZnCl2["ZnCl2"]
CultureMech_001875 --> ing16_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing16_ZnCl2 -.-> CHEBI_49976
ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001875 --> ing17_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
ing18_H3BO3["H3BO3"]
CultureMech_001875 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001875 --> ing19_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001875 --> ing20_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001875 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001875 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_NaOH["NaOH"]
CultureMech_001875 --> ing23_NaOH
CHEBI_32145["CHEBI:32145"]
ing23_NaOH -.-> CHEBI_32145
ing24_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001875 --> ing24_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing24_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing25_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001875 --> ing25_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing25_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing26_Biotin["Biotin"]
CultureMech_001875 --> ing26_Biotin
CHEBI_15956["CHEBI:15956"]
ing26_Biotin -.-> CHEBI_15956
ing27_Folic_acid["Folic acid"]
CultureMech_001875 --> ing27_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing27_Folic_acid -.-> CHEBI_27470
ing28_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001875 --> ing28_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing28_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing29_Thiamine_HCl["Thiamine HCl"]
CultureMech_001875 --> ing29_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing29_Thiamine_HCl -.-> CHEBI_49105
CultureMech_001875 --> more["...6 more"]
Ingredients (36)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
26.2605 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5.88235 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
7.14286 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.47059 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.756303 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.147059 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.262605 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.00052521 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Na-acetate x 3 H2O
C2H3O2.3H2O.Na 136.079 g/mol
|
250 G_PER_L |
CHEBI:32138
sodium acetate trihydrate
|
|
| 10 |
Disodium succinate
C4H4O4.2Na 162.052 g/mol
|
10 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 11 |
Isobutyric acid
C4H8O2 88.106 g/mol
|
0.19 G_PER_L |
CHEBI:16135
isobutyric acid
|
|
| 12 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
48 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 13 |
Na2CO3
CO3.2Na 105.988 g/mol
|
50 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 14 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
30 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 15 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 16 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 17 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 18 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 21 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 24 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 25 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 26 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 27 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 28 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 29 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 30 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 31 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 32 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 33 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 34 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 35 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 36 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic and reach a pH of around 6, then distribute under the same gas atmosphere into anoxic Hungate-type tubes and autoclave. Solutions B and C are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions D and F are autoclaved separately under 100% N2 gas. Solution E is prepared under 80% N2 and 20% CO2 gas gas atmosphere. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be at 7.0. After combining solutions the medium should equilibrate overnight and a white precipitate should be apparent.
- Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) just before inoculation may stimulate growth at the beginning. For transfers use 5 - 10% (v/v) inoculum.
- Adjust to pH 7.0 with 1 N NaOH.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.099851Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 739 |
| 2026-02-06T05:10:20.226441Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:08.035896Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001875 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:20.427931+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFONEMA ISHIMOTOI MEDIUM" to "desulfonema_ishimotoi_medium" for consistent matching |
| 2026-06-05T07:40:57.028447+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.738217+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |