dsic_medium_modified

CultureMech:001881 bacterial COMPLEX LIQUID
Original name: DSIC MEDIUM (modified)

Overview

External ontology
mediadive.medium:747 (source link unavailable) — DSIC MEDIUM (modified)
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 7.1}
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium747.pdf

Composition

flowchart LR
    CultureMech_001881["dsic_medium_modified"]
    ing0_NaCl["NaCl"]
    CultureMech_001881 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_NH4Cl["NH4Cl"]
    CultureMech_001881 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001881 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_K2SO4["K2SO4"]
    CultureMech_001881 --> ing3_K2SO4
    CHEBI_32036["CHEBI:32036"]
    ing3_K2SO4 -.-> CHEBI_32036
    ing4_Na_acetate["Na-acetate"]
    CultureMech_001881 --> ing4_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Na_acetate -.-> CHEBI_32954
    ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_001881 --> ing5_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_001881 --> ing6_Yeast_extract
    ing7_Vitamin_B12["Vitamin B12"]
    CultureMech_001881 --> ing7_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing7_Vitamin_B12 -.-> CHEBI_176843
    ing8_MOPS_buffer["MOPS buffer"]
    CultureMech_001881 --> ing8_MOPS_buffer
    CHEBI_44115["CHEBI:44115"]
    ing8_MOPS_buffer -.-> CHEBI_44115
    ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001881 --> ing9_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001881 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_NaHCO3["NaHCO3"]
    CultureMech_001881 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139
    ing12_HCl["HCl"]
    CultureMech_001881 --> ing12_HCl
    CHEBI_17883["CHEBI:17883"]
    ing12_HCl -.-> CHEBI_17883
    ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001881 --> ing13_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing14_ZnCl2["ZnCl2"]
    CultureMech_001881 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001881 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16_H3BO3["H3BO3"]
    CultureMech_001881 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001881 --> ing17_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001881 --> ing18_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001881 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001881 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
129.938 G_PER_L CHEBI:26710
sodium chloride
2 NH4Cl
Cl.H4N 53.492 g/mol
0.519751 G_PER_L CHEBI:31206
ammonium chloride
3 KH2PO4
H2O4P.K 136.084 g/mol
0.623701 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 K2SO4
2K.O4S 174.259 g/mol
2.59875 G_PER_L CHEBI:32036
potassium sulfate
5 Na-acetate
C2H3O2.Na 82.034 g/mol
2.079 G_PER_L CHEBI:32954
sodium acetate
6 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
0.10395 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
7 Yeast extract 0.779626 G_PER_L
8 Vitamin B12 1 G_PER_L CHEBI:176843
vitamin B12
9 MOPS buffer
C7H15NO4S 209.267 g/mol
2.18295 G_PER_L CHEBI:44115
3-(N-morpholino)propanesulfonic acid
10 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
142.857 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
11 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
2.85714 G_PER_L CHEBI:86158
calcium chloride dihydrate
12 NaHCO3
CHO3.Na 84.006 g/mol
83.3333 G_PER_L CHEBI:32139
sodium hydrogencarbonate
13 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
14 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
15 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
16 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
17 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
18 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
19 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. After cooling inject 1.0 ml of solution B and 0.2 ml of solution C in each tube of solution A. The pH of the complete medium is 7.0-7.1.
  2. The acronym MOPS stands for the organic buffer N-morpholinopropanesulfonic acid at pH 7.0. The pH of solution A is 7.0. Boil the medium under N2 gas and distribute about 13 ml in 15 ml Hungate tubes. Autoclave at 121°C for 15 minutes.
  3. pH 7.0.
  4. Boil medium under N2 gas and autoclave in a srew-capped bottle at 121°C for 15 minutes.
  5. Filter sterilize this solution.
  6. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.125522Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 747
2026-01-28T19:48:07.383963Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:08.104388Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001881
2026-03-17T06:29:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:20.504877+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DSIC MEDIUM (modified)" to "dsic_medium_modified" for consistent matching
2026-05-20T06:01:15.717126+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-7.1' -> {'min': 7.0, 'max': 7.1}
2026-06-05T07:40:58.832379+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.443822+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.