Overview
- External ontology
- mediadive.medium:748 (source link unavailable) — CENS MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium748.pdf
Composition
flowchart LR
CultureMech_001882["cens_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_001882 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_KH2PO4["KH2PO4"]
CultureMech_001882 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_001882 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_EDTA["EDTA"]
CultureMech_001882 --> ing3_EDTA
CHEBI_4735["CHEBI:4735"]
ing3_EDTA -.-> CHEBI_4735
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001882 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001882 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Vitamin_B12["Vitamin B12"]
CultureMech_001882 --> ing6_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing6_Vitamin_B12 -.-> CHEBI_176843
ing7_Biotin["Biotin"]
CultureMech_001882 --> ing7_Biotin
CHEBI_15956["CHEBI:15956"]
ing7_Biotin -.-> CHEBI_15956
ing8_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_001882 --> ing8_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing8_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing9_Soytone["Soytone"]
CultureMech_001882 --> ing9_Soytone
ing10_Na_pyruvate["Na-pyruvate"]
CultureMech_001882 --> ing10_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing10_Na_pyruvate -.-> CHEBI_50144
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001882 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_H3BO3["H3BO3"]
CultureMech_001882 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001882 --> ing13_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing14_ZnCl2["ZnCl2"]
CultureMech_001882 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001882 --> ing15_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
ing16_CoCl2_x_2_H2O["CoCl2 x 2 H2O"]
CultureMech_001882 --> ing16_CoCl2_x_2_H2O
CHEBI_35696["CHEBI:35696"]
ing16_CoCl2_x_2_H2O -.-> CHEBI_35696
ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001882 --> ing17_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
ing18_Na2SeO3["Na2SeO3"]
CultureMech_001882 --> ing18_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing18_Na2SeO3 -.-> CHEBI_48843
ing19_NaVO3_x_n_H2O["NaVO3 x n H2O"]
CultureMech_001882 --> ing19_NaVO3_x_n_H2O
CHEBI_132095["CHEBI:132095"]
ing19_NaVO3_x_n_H2O -.-> CHEBI_132095
ing20_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001882 --> ing20_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing20_FeCl2_x_4_H2O -.-> CHEBI_86249
ing21_HCl["HCl"]
CultureMech_001882 --> ing21_HCl
CHEBI_17883["CHEBI:17883"]
ing21_HCl -.-> CHEBI_17883
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.9 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
EDTA
C10H16N2O8 292.244 g/mol
|
12.22722 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
[Merged 3 duplicates: 0.005, 10.0, 2.22222] |
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.075 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 | Vitamin B12 | 2e-05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 8 |
Biotin
C10H16N2O3S 244.316 g/mol
|
1.5e-05 G_PER_L |
CHEBI:15956
biotin
|
|
| 9 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
0.5 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 10 | Soytone | 4 G_PER_L | ||
| 11 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
2.2 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.8 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.4 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.4 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.2 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 16 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.2 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 17 |
CoCl2 x 2 H2O
2Cl.Co 129.839 g/mol
|
0.08 G_PER_L |
CHEBI:35696
cobalt dichloride
|
|
| 18 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.04 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 19 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.02 G_PER_L |
CHEBI:48843
disodium selenite
|
|
| 20 |
NaVO3 x n H2O
H2O.Na.O3V 139.946 g/mol
|
0.02 G_PER_L |
CHEBI:132095
sodium metavanadate hydrate
|
|
| 21 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.11111 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 22 |
HCl
HCl 36.461 g/mol
|
3 G_PER_L |
CHEBI:17883
hydrogen chloride
|
Preparation
- Dissolve chemicals one at a time in approximately 200.0 ml distilled water. Bring final volume to 250.0 ml with additional water.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:12.128908Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 748 |
| 2026-01-28T19:48:07.509136Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:38.893318Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:08.115547Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001882 |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:20.516911+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CENS MEDIUM" to "cens_medium" for consistent matching |
| 2026-06-05T07:40:52.970366+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.431926+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |