sc_medium (CultureMech:001884)

Overview

External ontology

mediadive.medium:751 (source link unavailable) — SC MEDIUM

Applications

Microbial cultivation

pH

6.6

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium751.pdf Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The LB constituent expansion is withdrawn: the exact DSMZ formulation specifies bovine serum albumin instead. Source batch, stock and receiving-medium scopes are separate; chemical groundings and growth claims are not recertified.

Composition

flowchart LR
    CultureMech_001884["sc_medium"]
    ing0_Corn_meal_agar["Corn meal agar"]
    CultureMech_001884 --> ing0_Corn_meal_agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Corn_meal_agar -.-> CHEBI_2509
    ing1_Soy_peptone["Soy peptone"]
    CultureMech_001884 --> ing1_Soy_peptone
    ing2_K2HPO4["K2HPO4"]
    CultureMech_001884 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_KH2PO4["KH2PO4"]
    CultureMech_001884 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001884 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Distilled_water["Distilled water"]
    CultureMech_001884 --> ing5_Distilled_water

Ingredients (6)

#	Ingredient	Concentration	Ontology	Notes
1	Corn meal agar		CHEBI:2509 agar	Source batch input: 17 g. Final-medium volume is not established; withdraw the unsupported final g/L assertion without replacing it with an assumed one-liter value.
2	Soy peptone			Source batch input: 8 g. Final-medium volume is not established; withdraw the unsupported final g/L assertion without replacing it with an assumed one-liter value.
3	K2HPO4 HO4P.2K 174.174 g/mol		CHEBI:131527 dipotassium hydrogen phosphate	Source batch input: 1 g. Final-medium volume is not established; withdraw the unsupported final g/L assertion without replacing it with an assumed one-liter value.
4	KH2PO4 H2O4P.K 136.084 g/mol		CHEBI:63036 potassium dihydrogen phosphate	Source batch input: 1 g. Final-medium volume is not established; withdraw the unsupported final g/L assertion without replacing it with an assumed one-liter value.
5	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol		CHEBI:31795 magnesium sulfate heptahydrate	Source batch input: 0.2 g. Final-medium volume is not established; withdraw the unsupported final g/L assertion without replacing it with an assumed one-liter value.
6	Distilled water	1 L		Source water batch input: 1000 ml / 1000 = 1 L in this preparation only, not a measured final volume.

Preparation

Adjust the basal medium to pH 6.6 before autoclaving.
Autoclave the basal medium, including the hemin input; the source gives no fixed cycle temperature or duration.
Cool to 50 C, then add the separately filter-sterilized albumin, glucose and cysteine stocks.

Solutions

Hemin stock

Dose: 0.015 L

Stock composition
Ingredient	Concentration	Notes
Hemin		Stock label: 0.1% in 0.05N NaOH. Percentage basis is unspecified; no numeric stock strength or final-medium concentration is asserted.

Source batch dose: 15 ml / 1000 = 0.015 L per receiving batch, not g/L or a per-liter dose. Source product/stock qualifier: 0.1% in 0.05N NaOH.

NaOH solution (0.05 N)

Stock composition
Ingredient	Concentration	Notes
NaOH		Source solvent strength 0.05 N; normality is not converted to molarity.

Hemin-stock solvent only; fixed solvent dose and percentage basis are not supplied.

Bovine serum albumin stock

Dose: 0.01 L

Stock composition
Ingredient	Concentration	Notes
Bovine serum albumin		Stock label: 20%. Percentage basis is unspecified; no numeric stock strength or final-medium concentration is asserted. Bovine serum albumin is fraction V, not LB broth.

Source batch dose: 10 ml / 1000 = 0.01 L per receiving batch, not g/L or a per-liter dose. Source product/stock qualifier: 20%.

Glucose stock

Dose: 0.005 L

Stock composition
Ingredient	Concentration	Notes
Glucose		Stock label: 10%. Percentage basis is unspecified; no numeric stock strength or final-medium concentration is asserted.

Source batch dose: 5 ml / 1000 = 0.005 L per receiving batch, not g/L or a per-liter dose. Source product/stock qualifier: 10%.

Cysteine stock

Dose: 0.01 L

Stock composition
Ingredient	Concentration	Notes
Cysteine		Stock label: free base, 10%. Percentage basis is unspecified; no numeric stock strength or final-medium concentration is asserted.

Source batch dose: 10 ml / 1000 = 0.01 L per receiving batch, not g/L or a per-liter dose. Source product/stock qualifier: free base, 10%.

Target organisms

No target organisms are recorded.

Recipe variants

Name
Liquid SC medium
Relationship
PHYSICAL_STATE_VARIANT
Description
DSMZ 751 liquid formulation with altered phosphate batch inputs; not a sequential change to the agar base recipe.
Modifications
- Omit corn meal agar.
- Use KH2PO4 1.5 g per batch instead of 1 g.
- Use K2HPO4 0.5 g per batch instead of 1 g.
Purpose
Liquid medium
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium751.pdf
  Supports
  SUPPORT
  Snippet
  For liquid medium, omit corn meal agar and change the phosphate concentration to KH2PO4, 1.5 g and K2HPO4, 0.5 g.
  Explanation
  variants[0].modifications: All three modifications belong together. Grams per batch are not assumed grams per final liter. PDF page 1; layout extraction and page image inspected. No final-medium volume, percentage basis, density or chemical grounding is inferred. Locator=original recipe text; accessed=2026-10-08T21:53:37.797126+00:00; capture SHA256=4e923b20851eebb337f189949dd4ff7bc75ca8eb0309ae2ca9e4f7651b6673d3.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:12.138863Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 751
2026-03-13T06:19:08.138676Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:001884
2026-03-17T06:30:24.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:20.543624+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "SC MEDIUM" to "sc_medium" for consistent matching
2026-06-05T07:41:45.344277+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.326390+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-08T22:13:10.504331+00:00	codex	RESTORED_DSMZ_ALBUMIN_PREPARATIONS_AND_CONCENTRATION_SCOPES_WITH_EVIDENCE	Restore source-specific albumin, withdraw false LB inputs, separate preparation doses and stock compositions, and attach exact evidence for changed claims. Preserve IDs, unrelated groundings and earlier history. Unknown percentage bases and final-medium volume remain unknown.