Overview
- External ontology
- mediadive.medium:756c (source link unavailable) — AUTOTROPHIC NITROBACTER MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.4, 'max': 7.6}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium756c.pdf
Composition
flowchart LR
CultureMech_001891["autotrophic_nitrobacter_medium"]
ing0_NaNO2["NaNO2"]
CultureMech_001891 --> ing0_NaNO2
CHEBI_78870["CHEBI:78870"]
ing0_NaNO2 -.-> CHEBI_78870
ing1_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001891 --> ing1_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing1_MnSO4_x_H2O -.-> CHEBI_86364
ing2_H3BO3["H3BO3"]
CultureMech_001891 --> ing2_H3BO3
CHEBI_33118["CHEBI:33118"]
ing2_H3BO3 -.-> CHEBI_33118
ing3_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001891 --> ing3_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing3_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing4__NH4_6Mo7O24["(NH4)6Mo7O24"]
CultureMech_001891 --> ing4__NH4_6Mo7O24
CHEBI_91249["CHEBI:91249"]
ing4__NH4_6Mo7O24 -.-> CHEBI_91249
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001891 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001891 --> ing6_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing6_CuSO4_x_5_H2O -.-> CHEBI_31440
ing7_CaCO3["CaCO3"]
CultureMech_001891 --> ing7_CaCO3
CHEBI_3311["CHEBI:3311"]
ing7_CaCO3 -.-> CHEBI_3311
ing8_NaCl["NaCl"]
CultureMech_001891 --> ing8_NaCl
CHEBI_26710["CHEBI:26710"]
ing8_NaCl -.-> CHEBI_26710
ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001891 --> ing9_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
ing10_KH2PO4["KH2PO4"]
CultureMech_001891 --> ing10_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing10_KH2PO4 -.-> CHEBI_63036
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaNO2
NO2.Na 68.995 g/mol
|
2 G_PER_L |
CHEBI:78870
sodium nitrite
|
|
| 2 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.0338 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 3 |
H3BO3
H3BO3 61.833 g/mol
|
0.0494 G_PER_L |
CHEBI:33118
boric acid
|
|
| 4 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.0431 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 5 |
(NH4)6Mo7O24
2H4N.MoO4 196.014 g/mol
|
0.0371 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.0973 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.025 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 8 |
CaCO3
CO3.Ca 100.086 g/mol
|
0.07 G_PER_L |
CHEBI:3311
calcium carbonate
|
|
| 9 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 10 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 11 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Preparation
- Adjust pH to 8.6 with NaOH or KOH. Autoclave and leave the medium to stand for 2 - 3 days so that pH can adjust itself to pH 7.4 - 7.6.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.153071Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 756c |
| 2026-03-13T06:19:08.173125Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001891 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:20.585088+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AUTOTROPHIC NITROBACTER MEDIUM" to "autotrophic_nitrobacter_medium" for consistent matching |
| 2026-05-20T06:01:09.339820+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.4-7.6' -> {'min': 7.4, 'max': 7.6} |
| 2026-06-05T07:40:50.113405+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.655434+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |