halomonas_desiderata_medium

CultureMech:001895 bacterial DEFINED LIQUID
Original name: HALOMONAS DESIDERATA MEDIUM

Overview

External ontology
mediadive.medium:762 (source link unavailable) — HALOMONAS DESIDERATA MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 9.5, 'max': 10.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium762.pdf Preparation follow-up: water volumes are batch inputs, not measured final volumes. Original chemical mappings, history and source links are retained, not independently revalidated. Preparation follows the cited source; no dose into another receiving medium is inferred.

Composition

flowchart LR
    CultureMech_001895["halomonas_desiderata_medium"]
    ing0_Glucose["Glucose"]
    CultureMech_001895 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001895 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001895 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_KNO3["KNO3"]
    CultureMech_001895 --> ing3_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing3_KNO3 -.-> CHEBI_63043
    ing4_Distilled_water["Distilled water"]
    CultureMech_001895 --> ing4_Distilled_water

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Glucose
C6H12O6 180.156 g/mol
5 G_PER_L CHEBI:17234
glucose
 Source input: 5 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a concentration in a separately prepared base, a separate stock, or a measured final-medium volume.
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Source input: 0.2 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a concentration in a separately prepared base, a separate stock, or a measured final-medium volume.
3 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Source input: 1 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a concentration in a separately prepared base, a separate stock, or a measured final-medium volume.
4 KNO3
K.NO3 101.102 g/mol
2 G_PER_L CHEBI:63043
potassium nitrate
 Source input: 2 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a concentration in a separately prepared base, a separate stock, or a measured final-medium volume.
5 Distilled water 0.9 L mediadive.compound:4 (source link unavailable)
Distilled water
 900 ml distilled-water input for the base, separate from carbonate preparation water; not a final volume.

Preparation

  1. Prepare the medium without Na2CO3 and NaHCO3 in 900 ml distilled water and autoclave. Prepare the Na2CO3 and NaHCO3 in 100 ml water, sterilise separately and add to the cooled medium. Final pH 9.5-10.0.

Solutions

Separately sterilized carbonate preparation

Stock composition
IngredientConcentrationNotes
Na2CO3 Source batch input: 5.4 g Na2CO3 in the separately sterilized carbonate preparation. The former flattened g/L rate is withdrawn at this stock stage. The 100 ml water input does not establish final stock volume or a numeric stock dose.
NaHCO3 Source batch input: 4.2 g NaHCO3 in the separately sterilized carbonate preparation. The former flattened g/L rate is withdrawn at this stock stage. The 100 ml water input does not establish final stock volume or a numeric stock dose.
Distilled water 0.1 L 100 ml water input for the separate carbonate preparation, not final stock volume or stock dose.
100 ml water is input, not final stock volume or a numeric stock addition dose. Neither stock strength nor final-medium concentration is inferred; the separate sterilization method is unspecified.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.163380Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 762
2026-03-13T06:19:08.198459Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001895
2026-03-17T06:30:13.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:20.613391+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOMONAS DESIDERATA MEDIUM" to "halomonas_desiderata_medium" for consistent matching
2026-05-20T06:01:22.108701+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '9.5-10' -> {'min': 9.5, 'max': 10.0}
2026-06-05T07:41:07.868785+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.950950+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:59.548817+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T12:21:30.028294+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.
2026-10-08T04:23:42.348042+00:00 codex CORRECTED_DSMZ650_752_762_MEDIUM_AND_SOLUTION_PREPARATIONS Corrected source-explicit amounts and preparation stages; original excerpts and history retained. Earlier carbonate concentration claims explicitly withdrawn and replaced by scoped input-mass evidence. No new growth claim or chemical grounding.