isosphaera_pallida_medium

CultureMech:001898 bacterial COMPLEX LIQUID
Original name: ISOSPHAERA PALLIDA MEDIUM

Overview

External ontology
mediadive.medium:765 (source link unavailable) — ISOSPHAERA PALLIDA MEDIUM
Applications
  • Microbial cultivation
pH
7.9
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium765.pdf

Composition

flowchart LR
    CultureMech_001898["isosphaera_pallida_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001898 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001898 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001898 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001898 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_KCl["KCl"]
    CultureMech_001898 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_NaCl["NaCl"]
    CultureMech_001898 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_FeCl3["FeCl3"]
    CultureMech_001898 --> ing6_FeCl3
    CHEBI_30808["CHEBI:30808"]
    ing6_FeCl3 -.-> CHEBI_30808
    ing7_Glucose["Glucose"]
    CultureMech_001898 --> ing7_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing7_Glucose -.-> CHEBI_17234
    ing8_Casamino_acids["Casamino acids"]
    CultureMech_001898 --> ing8_Casamino_acids
    ing9_HCl["HCl"]
    CultureMech_001898 --> ing9_HCl
    CHEBI_17883["CHEBI:17883"]
    ing9_HCl -.-> CHEBI_17883
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001898 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_001898 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_ZnCl2["ZnCl2"]
    CultureMech_001898 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001898 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001898 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001898 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001898 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NaHCO3["NaHCO3"]
    CultureMech_001898 --> ing17_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing17_NaHCO3 -.-> CHEBI_32139
    ing18_Vitamin_B12["Vitamin B12"]
    CultureMech_001898 --> ing18_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing18_Vitamin_B12 -.-> CHEBI_176843
    ing19_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001898 --> ing19_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing19_Nicotinic_acid -.-> CHEBI_15940
    ing20_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001898 --> ing20_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing20_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001898 --> ing21_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing21_p_Aminobenzoic_acid -.-> CHEBI_30753

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1 G_PER_L CHEBI:62946
ammonium sulfate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.2 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 KCl
Cl.K 74.551 g/mol
4 G_PER_L CHEBI:32588
potassium chloride
6 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
7 FeCl3
Cl3Fe 162.204 g/mol
0.000292 G_PER_L CHEBI:30808
iron trichloride
8 Glucose
C6H12O6 180.156 g/mol
0.25 G_PER_L CHEBI:17234
glucose
9 Casamino acids 0.25 G_PER_L
10 HCl
HCl 36.461 g/mol
0.25 G_PER_L CHEBI:17883
hydrogen chloride
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.06 G_PER_L CHEBI:33118
boric acid
13 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.04 G_PER_L CHEBI:75213
sodium molybdate dihydrate
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.02 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 NaHCO3
CHO3.Na 84.006 g/mol
42 G_PER_L CHEBI:32139
sodium hydrogencarbonate
19 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
20 Nicotinic acid
C6H5NO2 123.111 g/mol
0.2 G_PER_L CHEBI:15940
nicotinic acid
21 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.1 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
22 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.002 G_PER_L CHEBI:30753
4-aminobenzoic acid

Preparation

  1. Prepare the medium by mixing 250 ml of solution A (without vitamins added) with 650 ml distilled water. Gas with air:CO2 (95:5 v/v), and fill the medium into serum bottles so that the gas to liquid ratio is about 5:1 (v/v). Gas the serum bottles with air:CO2 (95:5 v/v), seal the bottles as for anaerobic work and autoclave. To the cooled medium add (using a syringe) 10ml NaHCO3/ 90ml medium and 0.5ml sterile vitamin solution to 100ml of the resulting medium. Final pH 7.9.
  2. Fill into 100 ml serum bottle, gas with CO2 , seal as for anaerobic work and autoclave. Cool before adding to the final medium.
  3. Filter sterilise.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.170131Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 765
2026-01-28T19:48:28.802062Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:08.223170Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001898
2026-03-17T06:30:26.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:20.640720+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ISOSPHAERA PALLIDA MEDIUM" to "isosphaera_pallida_medium" for consistent matching
2026-06-05T07:41:09.639093+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:40.156864+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:47:00.137633+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.