Overview
- External ontology
- mediadive.medium:766 (source link unavailable) — THERMOCOCCUS CHITINOPHAGUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.7
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium766.pdf
Composition
flowchart LR
CultureMech_001899["thermococcus_chitinophagus_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_001899 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_NaHCO3["NaHCO3"]
CultureMech_001899 --> ing1_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing1_NaHCO3 -.-> CHEBI_32139
ing2_KH2PO4["KH2PO4"]
CultureMech_001899 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001899 --> ing3_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing3_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing4_Na2SeO4["Na2SeO4"]
CultureMech_001899 --> ing4_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing4_Na2SeO4 -.-> CHEBI_77775
ing5__NH4_2Ni_SO4_2["(NH4)2Ni(SO4)2"]
CultureMech_001899 --> ing5__NH4_2Ni_SO4_2
CHEBI_86149["CHEBI:86149"]
ing5__NH4_2Ni_SO4_2 -.-> CHEBI_86149
ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001899 --> ing6_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_x_9_H2O -.-> CHEBI_76209
ing7_Resazurin["Resazurin"]
CultureMech_001899 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Chitin["Chitin"]
CultureMech_001899 --> ing8_Chitin
CHEBI_17029["CHEBI:17029"]
ing8_Chitin -.-> CHEBI_17029
ing9_HCl["HCl"]
CultureMech_001899 --> ing9_HCl
CHEBI_17883["CHEBI:17883"]
ing9_HCl -.-> CHEBI_17883
ing10_NaCl["NaCl"]
CultureMech_001899 --> ing10_NaCl
CHEBI_26710["CHEBI:26710"]
ing10_NaCl -.-> CHEBI_26710
ing11_Na2SO4["Na2SO4"]
CultureMech_001899 --> ing11_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing11_Na2SO4 -.-> CHEBI_32149
ing12_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001899 --> ing12_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing12_MgCl2_x_6_H2O -.-> CHEBI_86345
ing13_CaCl2["CaCl2"]
CultureMech_001899 --> ing13_CaCl2
CHEBI_3312["CHEBI:3312"]
ing13_CaCl2 -.-> CHEBI_3312
ing14_KCl["KCl"]
CultureMech_001899 --> ing14_KCl
CHEBI_32588["CHEBI:32588"]
ing14_KCl -.-> CHEBI_32588
ing15_KBr["KBr"]
CultureMech_001899 --> ing15_KBr
CHEBI_32030["CHEBI:32030"]
ing15_KBr -.-> CHEBI_32030
ing16_H3BO3["H3BO3"]
CultureMech_001899 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_SrCl2["SrCl2"]
CultureMech_001899 --> ing17_SrCl2
CHEBI_36383["CHEBI:36383"]
ing17_SrCl2 -.-> CHEBI_36383
ing18_NaF["NaF"]
CultureMech_001899 --> ing18_NaF
CHEBI_28741["CHEBI:28741"]
ing18_NaF -.-> CHEBI_28741
ing19_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001899 --> ing19_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing19_Nitrilotriacetic_acid -.-> CHEBI_44557
ing20_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001899 --> ing20_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing20_MgSO4_x_7_H2O -.-> CHEBI_31795
ing21_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001899 --> ing21_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing21_MnSO4_x_H2O -.-> CHEBI_86364
ing22_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001899 --> ing22_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing22_FeSO4_x_7_H2O -.-> CHEBI_75836
ing23_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001899 --> ing23_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing23_CoSO4_x_7_H2O -.-> CHEBI_91244
ing24_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001899 --> ing24_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing24_CaCl2_x_2_H2O -.-> CHEBI_86158
ing25_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001899 --> ing25_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing25_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing26_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001899 --> ing26_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing26_CuSO4_x_5_H2O -.-> CHEBI_31440
ing27_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001899 --> ing27_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing27_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing28_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001899 --> ing28_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing28_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing29_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001899 --> ing29_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing29_NiCl2_x_6_H2O -.-> CHEBI_34887
CultureMech_001899 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.392 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
[Merged 2 duplicates: 0.2, 0.192] |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.00015 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 5 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.00015 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 6 |
(NH4)2Ni(SO4)2
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
0.0003 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 7 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Chitin
(C8H13NO5)n.H2O 221.209 g/mol
|
16.6667 G_PER_L |
CHEBI:17029
chitin
|
|
| 10 |
HCl
HCl 36.461 g/mol
|
74 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 11 |
NaCl
Cl.Na 58.443 g/mol
|
24.47 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 23.47, 1.0] |
| 12 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.92 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 13 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
10.64 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 14 |
CaCl2
Ca.2Cl 110.984 g/mol
|
1.1 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 15 |
KCl
Cl.K 74.551 g/mol
|
0.664 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 16 |
KBr
Br.K 119.002 g/mol
|
0.096 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.036 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.026, 0.01] |
| 18 |
SrCl2
2Cl.Sr 158.526 g/mol
|
0.024 G_PER_L |
CHEBI:36383
strontium dichloride
|
|
| 19 |
NaF
F.Na 41.988 g/mol
|
0.003 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 20 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 21 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 22 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 23 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 24 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 25 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 26 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 27 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 28 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 29 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 30 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 31 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Preparation
- Prepare the medium without Na2S x 9 H2O and NaHCO3, boil and cool under N2. Add the NaHCO3 to the cooled medium and adjust the pH to 6.7. Dispense into Hungate tubes or serum bottles under N2 and autoclave. Reduce the medium before use from a sterile stock solution of neutralised Na2S x 9 H2O.
- The chitin solution is prepared as follows: mix 20 g chitin (practical grade from crab shells) with 200 ml of 37% HCl (pre-cooled to 4°C) and stir for 1 hr at 4°C. Pour the suspension into 1 litre of distilled water (pre-cooled to 4°C) and filter through filter paper (Schleicher and Schüll Nr. 311853). Wash the residue five times with 500 ml distilled water and resuspend in 1 litre of distilled water. Neutralise the suspension with 10 ml of 5M KOH (final pH 6.5). Filter and wash with 3 litres of distilled water to remove KCl.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.173460Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 766 |
| 2026-03-13T03:09:07.590061Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:08.236670Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001899 |
| 2026-03-17T06:30:13.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:20.655980+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "THERMOCOCCUS CHITINOPHAGUS MEDIUM" to "thermococcus_chitinophagus_medium" for consistent matching |
| 2026-06-05T07:41:49.879454+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:36.014976+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:12.899602+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-20T19:00:59.608699+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |