Overview
- External ontology
- mediadive.medium:769 (source link unavailable) — CLOSTRIDIUM VINCENTII MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium769.pdf
Composition
flowchart LR
CultureMech_001902["clostridium_vincentii_medium"]
ing0_Sea_Salt["Sea Salt"]
CultureMech_001902 --> ing0_Sea_Salt
ing1_NH4NO3["NH4NO3"]
CultureMech_001902 --> ing1_NH4NO3
CHEBI_63038["CHEBI:63038"]
ing1_NH4NO3 -.-> CHEBI_63038
ing2_Yeast_extract["Yeast extract"]
CultureMech_001902 --> ing2_Yeast_extract
ing3_Trypticase_peptone["Trypticase peptone"]
CultureMech_001902 --> ing3_Trypticase_peptone
ing4_Sodium_resazurin["Sodium resazurin"]
CultureMech_001902 --> ing4_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Sodium_resazurin -.-> CHEBI_8806
ing5_NaHCO3["NaHCO3"]
CultureMech_001902 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_Na_phosphate_buffer["Na-phosphate buffer"]
CultureMech_001902 --> ing6_Na_phosphate_buffer
ing7_Lactose["Lactose"]
CultureMech_001902 --> ing7_Lactose
CHEBI_17716["CHEBI:17716"]
ing7_Lactose -.-> CHEBI_17716
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001902 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001902 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_Biotin["Biotin"]
CultureMech_001902 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_Folic_acid["Folic acid"]
CultureMech_001902 --> ing11_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing11_Folic_acid -.-> CHEBI_27470
ing12_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001902 --> ing12_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing12_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing13_Thiamine_HCl["Thiamine HCl"]
CultureMech_001902 --> ing13_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing13_Thiamine_HCl -.-> CHEBI_49105
ing14_Riboflavin["Riboflavin"]
CultureMech_001902 --> ing14_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing14_Riboflavin -.-> CHEBI_17015
ing15_Nicotinic_acid["Nicotinic acid"]
CultureMech_001902 --> ing15_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing15_Nicotinic_acid -.-> CHEBI_15940
ing16_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001902 --> ing16_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing16_Calcium_D_____pantothenate -.-> CHEBI_31345
ing17_Vitamin_B12["Vitamin B12"]
CultureMech_001902 --> ing17_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing17_Vitamin_B12 -.-> CHEBI_176843
ing18_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001902 --> ing18_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing18_p_Aminobenzoic_acid -.-> CHEBI_30753
ing19__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_001902 --> ing19__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing19__DL__alpha_Lipoic_acid -.-> CHEBI_16494
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea Salt | 11.988 G_PER_L | ||
| 2 |
NH4NO3
H4N.NO3 80.043 g/mol
|
0.0999001 G_PER_L |
CHEBI:63038
ammonium nitrate
|
|
| 3 | Yeast extract | 0.999001 G_PER_L | ||
| 4 | Trypticase peptone | 0.3996 G_PER_L | ||
| 5 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0004995 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.999001 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 | Na-phosphate buffer | 20 G_PER_L | ||
| 8 |
Lactose
C12H22O11 342.297 g/mol
|
1.998 G_PER_L |
CHEBI:17716
lactose
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.2997 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.2997 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 12 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 13 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 14 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 15 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 16 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 17 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 18 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 19 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 20 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients (except bicarbonate, phosphate-buffer, lactose, vitamins, cysteine and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add phosphate-buffer, lactose, cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of complete medium to 6.5, if necessary.
- Note: Sea salts can be replaced by 300.0 ml/l of filtered aged sea water.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.185782Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 769 |
| 2026-01-28T19:48:40.024423Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-06T05:10:51.893102Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:08.270616Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001902 |
| 2026-03-17T06:30:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:20.694363+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CLOSTRIDIUM VINCENTII MEDIUM" to "clostridium_vincentii_medium" for consistent matching |
| 2026-06-05T07:40:54.468256+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.900676+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |