Overview
- External ontology
- mediadive.medium:770 (source link unavailable) — SULFOPHOBOCOCCUS ZILLIGII MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium770.pdf
Composition
flowchart LR
CultureMech_001904["sulfophobococcus_zilligii_medium"]
ing0_Na2_EDTA["Na2-EDTA"]
CultureMech_001904 --> ing0_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing0_Na2_EDTA -.-> CHEBI_64734
ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001904 --> ing1_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001904 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_KCl["KCl"]
CultureMech_001904 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_ZnCl2["ZnCl2"]
CultureMech_001904 --> ing4_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing4_ZnCl2 -.-> CHEBI_49976
ing5_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001904 --> ing5_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing5_MnSO4_x_H2O -.-> CHEBI_86364
ing6_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_001904 --> ing6_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing6_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing7_Glycine["Glycine"]
CultureMech_001904 --> ing7_Glycine
CHEBI_15428["CHEBI:15428"]
ing7_Glycine -.-> CHEBI_15428
ing8_Sodium_resazurin["Sodium resazurin"]
CultureMech_001904 --> ing8_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Sodium_resazurin -.-> CHEBI_8806
ing9_Na2CO3["Na2CO3"]
CultureMech_001904 --> ing9_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing9_Na2CO3 -.-> CHEBI_29377
ing10_Yeast_extract["Yeast extract"]
CultureMech_001904 --> ing10_Yeast_extract
ing11_Trypticase_peptone["Trypticase peptone"]
CultureMech_001904 --> ing11_Trypticase_peptone
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001904 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_H2SO4["H2SO4"]
CultureMech_001904 --> ing13_H2SO4
CHEBI_26836["CHEBI:26836"]
ing13_H2SO4 -.-> CHEBI_26836
ing14_Dithiothreitol["Dithiothreitol"]
CultureMech_001904 --> ing14_Dithiothreitol
CHEBI_18320["CHEBI:18320"]
ing14_Dithiothreitol -.-> CHEBI_18320
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.0329897 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 2 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0680412 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.0319588 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.0319588 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.0021 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 6 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.0023 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 7 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.0018 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
|
| 8 |
Glycine
C2H5NO2 75.067 g/mol
|
1.54639 G_PER_L |
CHEBI:15428
glycine
|
|
| 9 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.237113 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 11 | Yeast extract | 100 G_PER_L | ||
| 12 | Trypticase peptone | 100 G_PER_L | ||
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
10 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
H2SO4
H2O4S 98.079 g/mol
|
5 G_PER_L |
CHEBI:26836
sulfuric acid
|
|
| 15 |
Dithiothreitol
C4H10O2S2 154.256 g/mol
|
15 G_PER_L |
CHEBI:18320
1,4-dithiothreitol
|
Preparation
- Dissolve ingredients of solution A except carbonate, sparge with 100% N2 gas for 30 - 45 min to make it anoxic, then add carbonate and adjust pH to 9.0 - 9.3. Dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials (e.g., 30 ml medium per 100 ml bottle) and autoclave. Solutions B and C are autoclaved separately under 100% N2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and sterilized by filtration. To complete the medium add appropriate amounts of solutions B to E to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.5.
- Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning.
- For transfers use 10% (v/v) inoculum.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.188927Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 770 |
| 2026-03-13T06:19:08.289242Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001904 |
| 2026-03-17T06:29:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:20.715203+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SULFOPHOBOCOCCUS ZILLIGII MEDIUM" to "sulfophobococcus_zilligii_medium" for consistent matching |
| 2026-06-05T07:41:47.525624+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:12.051843+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |