Overview
- External ontology
- mediadive.medium:775 (source link unavailable) — R - CW MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium775.pdf
Composition
flowchart LR
CultureMech_001909["r_cw_medium"]
ing0_Trypticase["Trypticase"]
CultureMech_001909 --> ing0_Trypticase
ing1_Tryptone["Tryptone"]
CultureMech_001909 --> ing1_Tryptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_001909 --> ing2_Yeast_extract
ing3_KH2PO4["KH2PO4"]
CultureMech_001909 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_Na_acetate["Na-acetate"]
CultureMech_001909 --> ing4_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing4_Na_acetate -.-> CHEBI_32954
ing5__NH4_2_citrate["(NH4)2 citrate"]
CultureMech_001909 --> ing5__NH4_2_citrate
CHEBI_63076["CHEBI:63076"]
ing5__NH4_2_citrate -.-> CHEBI_63076
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001909 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001909 --> ing7_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing7_MnSO4_x_H2O -.-> CHEBI_86364
ing8_Tween_80["Tween 80"]
CultureMech_001909 --> ing8_Tween_80
CHEBI_53426["CHEBI:53426"]
ing8_Tween_80 -.-> CHEBI_53426
ing9_Cheese_whey["Cheese whey"]
CultureMech_001909 --> ing9_Cheese_whey
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase | 5 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
|
| 2 | Tryptone | 5 G_PER_L | ||
| 3 | Yeast extract | 5 G_PER_L | ||
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
5 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 6 |
(NH4)2 citrate
C6H6O7.2H4N 226.185 g/mol
|
2 G_PER_L |
CHEBI:63076
diammonium citrate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.2 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 9 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
|
| 10 | Cheese whey | 1000 G_PER_L |
Preparation
- Adjust pH to 5.5 and distribute into screw-capped Wheaton tubes or Hungate tubes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.202540Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 775 |
| 2026-01-28T19:48:08.006319Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:08.328611Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001909 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:20.759218+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R - CW MEDIUM" to "r_cw_medium" for consistent matching |
| 2026-06-05T07:41:43.784047+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.830012+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-22T00:03:26.686152+00:00 | repair_rejected_peptone_groundings.py | CORRECTED_REJECTED_PEPTONE_GROUNDING | CultureMech #484. /ingredients/0/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved. |
| 2026-10-07T12:21:30.028294+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |