capnocytophaga_ii_medium

CultureMech:001914 bacterial COMPLEX SOLID_AGAR
Original name: CAPNOCYTOPHAGA II MEDIUM

Overview

External ontology
mediadive.medium:779 (source link unavailable) — CAPNOCYTOPHAGA II MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.6, 'max': 7.8}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium779.pdf

Composition

flowchart LR
    CultureMech_001914["capnocytophaga_ii_medium"]
    ing0_Lab_Lemco_beef_extract["Lab-Lemco beef extract"]
    CultureMech_001914 --> ing0_Lab_Lemco_beef_extract
    ing1_Proteose_peptone_no__3["Proteose peptone no. 3"]
    CultureMech_001914 --> ing1_Proteose_peptone_no__3
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_001914 --> ing2_Yeast_extract
    ing3_Na2HPO4["Na2HPO4"]
    CultureMech_001914 --> ing3_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing3_Na2HPO4 -.-> CHEBI_34683
    ing4_Glucose["Glucose"]
    CultureMech_001914 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Starch["Starch"]
    CultureMech_001914 --> ing5_Starch
    CHEBI_28017["CHEBI:28017"]
    ing5_Starch -.-> CHEBI_28017
    ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001914 --> ing6_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing7_Agar["Agar"]
    CultureMech_001914 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Horse_blood["Horse blood"]
    CultureMech_001914 --> ing8_Horse_blood

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Lab-Lemco beef extract 2.4 G_PER_L
2 Proteose peptone no. 3 10 G_PER_L
3 Yeast extract 5 G_PER_L
4 Na2HPO4
HO4P.2Na 141.958 g/mol
4 G_PER_L CHEBI:34683
disodium hydrogenphosphate
5 Glucose
C6H12O6 180.156 g/mol
1.5 G_PER_L CHEBI:17234
glucose
6 Starch 0.5 G_PER_L CHEBI:28017
starch
7 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
8 Agar 15 G_PER_L CHEBI:2509
agar
(if necessary)
9 Horse blood 50 G_PER_L

Preparation

  1. Adjust pH to 7.6 - 7.8.
  2. Add the horse blood to the heat sterilized medium after being cooled to 50°C. Incubate under air plus 5% CO2 or anaerobically under 95% N2 plus 5% CO2.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.219008Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 779
2026-03-13T06:19:08.382287Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001914
2026-03-17T06:29:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:20.817878+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CAPNOCYTOPHAGA II MEDIUM" to "capnocytophaga_ii_medium" for consistent matching
2026-05-20T06:01:10.913924+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.6-7.8' -> {'min': 7.6, 'max': 7.8}
2026-06-05T07:40:52.485496+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.