middlebrook_medium_with_mycobactin

Overview

External ontology

mediadive.medium:780 (source link unavailable) — MIDDLEBROOK MEDIUM WITH MYCOBACTIN

Applications

• Microbial cultivation

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium780.pdf

Composition

flowchart LR
    CultureMech_001916["middlebrook_medium_with_mycobactin"]
    ing0_Mycobactine_J["Mycobactine J"]
    CultureMech_001916 --> ing0_Mycobactine_J
    ing1_Absolute_ethanol["Absolute ethanol"]
    CultureMech_001916 --> ing1_Absolute_ethanol
    ing2_Bacto_Middlebrook_7H10_agar["Bacto Middlebrook 7H10 agar"]
    CultureMech_001916 --> ing2_Bacto_Middlebrook_7H10_agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Bacto_Middlebrook_7H10_agar -.-> CHEBI_2509
    ing3_Glycerol["Glycerol"]
    CultureMech_001916 --> ing3_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing3_Glycerol -.-> CHEBI_17754

Ingredients (4)

Preparation

1. Reconstitute the contents of one vial of 2 mg Mycobactine J with 2 ml absolute ethanol (analytical grade). Shake carefully so that the product is completely dissolved. Pour the contents of the bottle into 1 litre of medium 645 kept melted in a water bath at +56°C, taking care to pour out all of the product. Rinse out the vial that contained the Mycobactine J with an additional 2 ml of absolute ethanol and pour into the culture medium. Mix the Mycobactine J alcohol solution and the culture medium very thoroughly.
2. Sterilize for 15 min by 121°C cool to 50 - 55°C. Add 100 ml Middlebrook OADC enrichment (Becton Dickinson 212240) under aseptic conditions and mix thoroughly.
3. If you can´t order the commercial media then prepare the media in this way:

Target organisms

No target organisms are recorded.

Provenance