dung_extract_agar

CultureMech:001917 bacterial COMPLEX SOLID_AGAR
Original name: DUNG EXTRACT AGAR

Overview

External ontology
mediadive.medium:781 (source link unavailable) — DUNG EXTRACT AGAR
Applications
  • Microbial cultivation
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium781.pdf

Composition

flowchart LR
    CultureMech_001917["dung_extract_agar"]
    ing0_Dung_extract["Dung extract"]
    CultureMech_001917 --> ing0_Dung_extract
    ing1_Malt_extract["Malt extract"]
    CultureMech_001917 --> ing1_Malt_extract
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001917 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_001917 --> ing3_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing3_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing4_K2HPO4["K2HPO4"]
    CultureMech_001917 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Peptone["Peptone"]
    CultureMech_001917 --> ing5_Peptone
    ing6_Agar["Agar"]
    CultureMech_001917 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Dung extract 100 G_PER_L
2 Malt extract 5 G_PER_L
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
0.72 G_PER_L CHEBI:86159
calcium nitrate tetrahydrate
5 K2HPO4
HO4P.2K 174.174 g/mol
0.25 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 Peptone 0.1 G_PER_L
7 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Dung extract: Boil an average sized piece of horse dung (fresh or frozen) for two hours in 150 ml water using a water bath; filter and use immediately. Sterilize at 121°C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.222356Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 781
2026-03-13T06:19:08.392708Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001917
2026-03-17T06:30:18.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:20.830389+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DUNG EXTRACT AGAR" to "dung_extract_agar" for consistent matching
2026-06-05T07:40:59.166162+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.575344+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T12:21:30.028294+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.