om_2_medium

CultureMech:001918 bacterial DEFINED SOLID_AGAR
Original name: OM-2 MEDIUM

Overview

External ontology
mediadive.medium:782 (source link unavailable) — OM-2 MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 8.5, 'max': 9.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium782.pdf

Composition

flowchart LR
    CultureMech_001918["om_2_medium"]
    ing0_NH4_oxalate["NH4-oxalate"]
    CultureMech_001918 --> ing0_NH4_oxalate
    CHEBI_91241["CHEBI:91241"]
    ing0_NH4_oxalate -.-> CHEBI_91241
    ing1_NaHCO3["NaHCO3"]
    CultureMech_001918 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
    CultureMech_001918 --> ing2_NaH2PO4_x_2_H2O
    CHEBI_37585["CHEBI:37585"]
    ing2_NaH2PO4_x_2_H2O -.-> CHEBI_37585
    ing3_NaCl["NaCl"]
    CultureMech_001918 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KCl["KCl"]
    CultureMech_001918 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001918 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001918 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_001918 --> ing7_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing8_Agar["Agar"]
    CultureMech_001918 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 NH4-oxalate
C2O4.2H4N 124.096 g/mol
15 G_PER_L CHEBI:91241
ammonium oxalate
2 NaHCO3
CHO3.Na 84.006 g/mol
10 G_PER_L CHEBI:32139
sodium hydrogencarbonate
3 NaH2PO4 x 2 H2O
H2O4P.Na 119.976 g/mol
10 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
4 NaCl
Cl.Na 58.443 g/mol
0.7 G_PER_L CHEBI:26710
sodium chloride
5 KCl
Cl.K 74.551 g/mol
0.57 G_PER_L CHEBI:32588
potassium chloride
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.01 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
1 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
9 Agar 20 G_PER_L CHEBI:2509
agar
 (if required)

Preparation

  1. pH 6.8-7.0
  2. The first three salts must be dissolved in the indicated sequence. After autoclaving for 20 min at 121°C the pH of the medium shifted to 8.5-9.0. Solid medium contained 1.5 to 2.0% (w/v) of Bacto-Agar (Difco).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.223837Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 782
2026-03-13T06:19:08.397913Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001918
2026-03-17T06:29:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:20.836017+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "OM-2 MEDIUM" to "om_2_medium" for consistent matching
2026-05-20T06:02:01.528482+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '8.5-9' -> {'min': 8.5, 'max': 9.0}
2026-06-05T07:41:40.459427+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.752906+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T12:21:30.028294+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.