chopped_meat_medium_for_fretibacterium_sp

Overview

External ontology

mediadive.medium:78c (source link unavailable) — CHOPPED MEAT MEDIUM FOR FRETIBACTERIUM SP.

Applications

• Microbial cultivation

pH

7.0

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium78c.pdf Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_001929["chopped_meat_medium_for_fretibacterium_sp"]
    ing0_Ground_beef["Ground beef"]
    CultureMech_001929 --> ing0_Ground_beef
    ing1_NaOH["NaOH"]
    CultureMech_001929 --> ing1_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing1_NaOH -.-> CHEBI_32145
    ing2_Casein_peptone["Casein peptone"]
    CultureMech_001929 --> ing2_Casein_peptone
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_001929 --> ing3_Yeast_extract
    ing4_K2HPO4["K2HPO4"]
    CultureMech_001929 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001929 --> ing5_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Sodium_resazurin -.-> CHEBI_8806
    ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001929 --> ing6_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing7_Na_fumarate["Na-fumarate"]
    CultureMech_001929 --> ing7_Na_fumarate
    CHEBI_115156["CHEBI:115156"]
    ing7_Na_fumarate -.-> CHEBI_115156
    ing8_Na_formate["Na-formate"]
    CultureMech_001929 --> ing8_Na_formate
    CHEBI_62965["CHEBI:62965"]
    ing8_Na_formate -.-> CHEBI_62965
    ing9_Horse_serum["Horse serum"]
    CultureMech_001929 --> ing9_Horse_serum
    ing10_DL_Dithiothreitol["DL-Dithiothreitol"]
    CultureMech_001929 --> ing10_DL_Dithiothreitol
    CHEBI_18320["CHEBI:18320"]
    ing10_DL_Dithiothreitol -.-> CHEBI_18320
    ing11_Isobutyric_acid["Isobutyric acid"]
    CultureMech_001929 --> ing11_Isobutyric_acid
    CHEBI_16135["CHEBI:16135"]
    ing11_Isobutyric_acid -.-> CHEBI_16135
    ing12_Tryptone["Tryptone"]
    CultureMech_001929 --> ing12_Tryptone
    ing13_Yeast_extract["Yeast extract"]
    CultureMech_001929 --> ing13_Yeast_extract
    ing14_Sodium_chloride["Sodium chloride"]
    CultureMech_001929 --> ing14_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing14_Sodium_chloride -.-> CHEBI_26710
    ing15_Valeric_acid["Valeric acid"]
    CultureMech_001929 --> ing15_Valeric_acid
    CHEBI_17418["CHEBI:17418"]
    ing15_Valeric_acid -.-> CHEBI_17418
    ing16_Isovaleric_acid["Isovaleric acid"]
    CultureMech_001929 --> ing16_Isovaleric_acid
    CHEBI_28484["CHEBI:28484"]
    ing16_Isovaleric_acid -.-> CHEBI_28484
    ing17_Haemin["Haemin"]
    CultureMech_001929 --> ing17_Haemin
    CHEBI_50385["CHEBI:50385"]
    ing17_Haemin -.-> CHEBI_50385
    ing18_Vitamin_K1["Vitamin K1"]
    CultureMech_001929 --> ing18_Vitamin_K1
    CHEBI_18067["CHEBI:18067"]
    ing18_Vitamin_K1 -.-> CHEBI_18067
    ing19_Ethanol["Ethanol"]
    CultureMech_001929 --> ing19_Ethanol
    CHEBI_16236["CHEBI:16236"]
    ing19_Ethanol -.-> CHEBI_16236
    ing20_Biotin["Biotin"]
    CultureMech_001929 --> ing20_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing20_Biotin -.-> CHEBI_15956
    ing21_Folic_acid["Folic acid"]
    CultureMech_001929 --> ing21_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing21_Folic_acid -.-> CHEBI_27470
    ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001929 --> ing22_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing23_Thiamine_HCl["Thiamine HCl"]
    CultureMech_001929 --> ing23_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing23_Thiamine_HCl -.-> CHEBI_49105
    ing24_Riboflavin["Riboflavin"]
    CultureMech_001929 --> ing24_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing24_Riboflavin -.-> CHEBI_17015
    ing25_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001929 --> ing25_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing25_Nicotinic_acid -.-> CHEBI_15940
    ing26_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_001929 --> ing26_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing26_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing27_Vitamin_B12["Vitamin B12"]
    CultureMech_001929 --> ing27_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing27_Vitamin_B12 -.-> CHEBI_176843
    ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001929 --> ing28_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing29__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_001929 --> ing29__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing29__DL__alpha_Lipoic_acid -.-> CHEBI_16494

Ingredients (30)

Preparation

1. Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add Casitone, yeast extract, hydrogen phosphate and resazurin, then add water to a final volume of 700 ml. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then add cysteine and adjust pH to 7.0. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes containing meat particles (use ca. 7 ml liquid medium for 1 - 2 g meat particles), then autoclave at 121°C for 20 min. After autoclaving add fumarate, formate, rumen fluid, horse serum, fatty acids, vitamins, haemin, vitamin K1 and DTT rom sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and sterilized by filtration.
2. Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C.
3. Adjust pH to 7.5 with concentrated NaOH.
4. Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.
5. Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.

Target organisms

No target organisms are recorded.

Provenance