porphyrobacter_tepidarius_medium

CultureMech:001931 bacterial COMPLEX LIQUID
Original name: PORPHYROBACTER TEPIDARIUS MEDIUM

Overview

External ontology
mediadive.medium:791 (source link unavailable) — PORPHYROBACTER TEPIDARIUS MEDIUM
Applications
  • Microbial cultivation
pH
7.5
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium791.pdf

Composition

flowchart LR
    CultureMech_001931["porphyrobacter_tepidarius_medium"]
    ing0_Na_glutamate["Na glutamate"]
    CultureMech_001931 --> ing0_Na_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing0_Na_glutamate -.-> CHEBI_64220
    ing1_Sodium_succinate["Sodium succinate"]
    CultureMech_001931 --> ing1_Sodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing1_Sodium_succinate -.-> CHEBI_63675
    ing2_Sodium_acetate["Sodium acetate"]
    CultureMech_001931 --> ing2_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing2_Sodium_acetate -.-> CHEBI_32954
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_001931 --> ing3_Yeast_extract
    ing4_Casamino_acids["Casamino acids"]
    CultureMech_001931 --> ing4_Casamino_acids
    ing5__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001931 --> ing5__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing5__NH4_2SO4 -.-> CHEBI_62946
    ing6_Sodium_thiosulphate["Sodium thiosulphate"]
    CultureMech_001931 --> ing6_Sodium_thiosulphate
    CHEBI_132112["CHEBI:132112"]
    ing6_Sodium_thiosulphate -.-> CHEBI_132112
    ing7_Na3_EDTA["Na3-EDTA"]
    CultureMech_001931 --> ing7_Na3_EDTA
    CHEBI_63125["CHEBI:63125"]
    ing7_Na3_EDTA -.-> CHEBI_63125
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001931 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001931 --> ing9_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001931 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_NaCl["NaCl"]
    CultureMech_001931 --> ing11_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing11_NaCl -.-> CHEBI_26710
    ing12_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
    CultureMech_001931 --> ing12_MnSO4_x_4_H2O
    CHEBI_86358["CHEBI:86358"]
    ing12_MnSO4_x_4_H2O -.-> CHEBI_86358
    ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001931 --> ing13_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing14_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_001931 --> ing14_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing14_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001931 --> ing15_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing16_Na2Mo4_x_2_H2O["Na2Mo4 x 2 H2O"]
    CultureMech_001931 --> ing16_Na2Mo4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2Mo4_x_2_H2O -.-> CHEBI_75213
    ing17_H3BO3["H3BO3"]
    CultureMech_001931 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_KH2PO4["KH2PO4"]
    CultureMech_001931 --> ing18_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing18_KH2PO4 -.-> CHEBI_63036
    ing19_K2HPO4["K2HPO4"]
    CultureMech_001931 --> ing19_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing19_K2HPO4 -.-> CHEBI_131527
    ing20_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001931 --> ing20_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing20_Nicotinic_acid -.-> CHEBI_15940
    ing21_Thiamine["Thiamine"]
    CultureMech_001931 --> ing21_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing21_Thiamine -.-> CHEBI_18385
    ing22_Biotin["Biotin"]
    CultureMech_001931 --> ing22_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing22_Biotin -.-> CHEBI_15956
    ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001931 --> ing23_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing24_Vitamin_B12["Vitamin B12"]
    CultureMech_001931 --> ing24_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing24_Vitamin_B12 -.-> CHEBI_176843
    ing25_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001931 --> ing25_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing25_Calcium_pantothenate -.-> CHEBI_31345
    ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001931 --> ing26_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing27_Folic_acid["Folic acid"]
    CultureMech_001931 --> ing27_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing27_Folic_acid -.-> CHEBI_27470

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 Na glutamate
C5H8NO4.Na 169.112 g/mol
0.5 G_PER_L CHEBI:64220
monosodium glutamate
2 Sodium succinate
C4H4O4.2Na 162.052 g/mol
0.5 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
3 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.5 G_PER_L CHEBI:32954
sodium acetate
4 Yeast extract 0.5 G_PER_L
5 Casamino acids 0.5 G_PER_L
6 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
7 Sodium thiosulphate
2Na.O3S2 158.111 g/mol
0.5 G_PER_L CHEBI:132112
sodium thiosulfate
8 Na3-EDTA
C10H13N2O8.3Na 358.19 g/mol
6.53 G_PER_L CHEBI:63125
EDTA trisodium salt
 [Merged 2 duplicates: 4.53, 2.0]
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.11 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
10 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
24.65 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
11 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
2.94 G_PER_L CHEBI:86158
calcium chloride dihydrate
12 NaCl
Cl.Na 58.443 g/mol
23.4 G_PER_L CHEBI:26710
sodium chloride
13 MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
0.112 G_PER_L CHEBI:86358
manganese(II) sulfate tetrahydrate
14 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.029 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
15 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.029 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
16 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.025 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
17 Na2Mo4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.024 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 H3BO3
H3BO3 61.833 g/mol
0.031 G_PER_L CHEBI:33118
boric acid
19 KH2PO4
H2O4P.K 136.084 g/mol
75 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
20 K2HPO4
HO4P.2K 174.174 g/mol
78 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
21 Nicotinic acid
C6H5NO2 123.111 g/mol
1 G_PER_L CHEBI:15940
nicotinic acid
22 Thiamine
C12H17N4OS 265.362 g/mol
1 G_PER_L CHEBI:18385
thiamine(1+)
23 Biotin
C10H16N2O3S 244.316 g/mol
0.05 G_PER_L CHEBI:15956
biotin
24 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.5 G_PER_L CHEBI:30753
4-aminobenzoic acid
25 Vitamin B12 0.01 G_PER_L CHEBI:176843
vitamin B12
26 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.5 G_PER_L CHEBI:31345
Calcium pantothenate
27 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.5 G_PER_L CHEBI:30961
pyridoxine hydrochloride
28 Folic acid
C19H19N7O6 441.404 g/mol
0.5 G_PER_L CHEBI:27470
folic acid

Preparation

  1. Final pH 7.5
  2. Add the vitamin solution, aseptically to the cooled, autoclaved medium.
  3. Filter sterilize.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.249778Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 791
2026-01-28T19:48:39.063655Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:37.457289Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:08.542139Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001931
2026-03-17T06:30:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:20.994164+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PORPHYROBACTER TEPIDARIUS MEDIUM" to "porphyrobacter_tepidarius_medium" for consistent matching
2026-06-05T07:41:42.356175+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:49.798222+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:47:10.354575+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.