Overview
- External ontology
- mediadive.medium:792c (source link unavailable) — NAUTILIA NITRATIREDUCENS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.5
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium792c.pdf
Composition
flowchart LR
CultureMech_001935["nautilia_nitratireducens_medium"]
ing0_NaCl["NaCl"]
CultureMech_001935 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001935 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001935 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001935 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KCl["KCl"]
CultureMech_001935 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaBr["NaBr"]
CultureMech_001935 --> ing5_NaBr
CHEBI_63004["CHEBI:63004"]
ing5_NaBr -.-> CHEBI_63004
ing6_H3BO3["H3BO3"]
CultureMech_001935 --> ing6_H3BO3
CHEBI_33118["CHEBI:33118"]
ing6_H3BO3 -.-> CHEBI_33118
ing7_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_001935 --> ing7_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing7_SrCl2_x_6_H2O -.-> CHEBI_36385
ing8_KI["KI"]
CultureMech_001935 --> ing8_KI
CHEBI_8346["CHEBI:8346"]
ing8_KI -.-> CHEBI_8346
ing9_KNO3["KNO3"]
CultureMech_001935 --> ing9_KNO3
CHEBI_63043["CHEBI:63043"]
ing9_KNO3 -.-> CHEBI_63043
ing10_KH2PO4["KH2PO4"]
CultureMech_001935 --> ing10_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing10_KH2PO4 -.-> CHEBI_63036
ing11_Na2CO3["Na2CO3"]
CultureMech_001935 --> ing11_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing11_Na2CO3 -.-> CHEBI_29377
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001935 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001935 --> ing13_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
ing14_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001935 --> ing14_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing14_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001935 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_001935 --> ing16__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing16__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing17_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001935 --> ing17_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing17_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing18_Na2SeO4["Na2SeO4"]
CultureMech_001935 --> ing18_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing18_Na2SeO4 -.-> CHEBI_77775
ing19_Biotin["Biotin"]
CultureMech_001935 --> ing19_Biotin
CHEBI_15956["CHEBI:15956"]
ing19_Biotin -.-> CHEBI_15956
ing20_Folic_acid["Folic acid"]
CultureMech_001935 --> ing20_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing20_Folic_acid -.-> CHEBI_27470
ing21_Thiamine_HCl["Thiamine HCl"]
CultureMech_001935 --> ing21_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing21_Thiamine_HCl -.-> CHEBI_49105
ing22_Riboflavin["Riboflavin"]
CultureMech_001935 --> ing22_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing22_Riboflavin -.-> CHEBI_17015
ing23_Nicotinic_acid["Nicotinic acid"]
CultureMech_001935 --> ing23_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing23_Nicotinic_acid -.-> CHEBI_15940
ing24_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001935 --> ing24_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing24_Calcium_D_____pantothenate -.-> CHEBI_31345
ing25_Vitamin_B12["Vitamin B12"]
CultureMech_001935 --> ing25_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing25_Vitamin_B12 -.-> CHEBI_176843
ing26_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001935 --> ing26_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing26_p_Aminobenzoic_acid -.-> CHEBI_30753
ing27__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_001935 --> ing27__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing27__DL__alpha_Lipoic_acid -.-> CHEBI_16494
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
30.8768 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 20.8768, 10.0] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
33.65344 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.65344, 30.0] |
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.87056 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.7828810000000002 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.782881, 1.0] |
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.344468 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
NaBr
Br.Na 102.894 g/mol
|
0.0521921 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 7 |
H3BO3
H3BO3 61.833 g/mol
|
0.1156576 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.0156576, 0.1] |
| 8 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.00730689 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 9 |
KI
I.K 166.002 g/mol
|
5.21921e-05 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 10 |
KNO3
K.NO3 101.102 g/mol
|
100 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 11 |
KH2PO4
H2O4P.K 136.084 g/mol
|
50 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 12 |
Na2CO3
CO3.2Na 105.988 g/mol
|
50 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
30 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 14 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 15 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.18 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2.8 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 18 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 19 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.1 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 20 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 21 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 22 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 23 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 24 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 25 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 26 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 27 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 28 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Sparge solution A with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic serum vials (e.g., 20 ml medium in 100 ml bottles) and autoclave. Solutions B and C are autoclaved separately under 80% N2 and 20% CO2 gas mixture. Solution D is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solution E is autoclaved under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B to E are added to solution A. Adjust pH of the complete medium to 6.5, if necessary.
- After inoculation pressurize vials to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
- Note: Solution A has to be prepared freshly. It cannot be stored for a long period of time.
- First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.
Solutions
Wolfe's mineral elixir
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MnSO4 x H2O | 5 G_PER_L | |
| FeSO4 x 7 H2O | 1 G_PER_L | |
| CoCl2 x 6 H2O | 1.8 G_PER_L | |
| ZnSO4 x 7 H2O | 1.8 G_PER_L |
Wolin's vitamin solution (10x)
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Pyridoxine hydrochloride | 0.1 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.266820Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 792c |
| 2026-02-06T05:10:13.464348Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:08:39.935409Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:08.596001Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001935 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:21.053768+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NAUTILIA NITRATIREDUCENS MEDIUM" to "nautilia_nitratireducens_medium" for consistent matching |
| 2026-06-05T07:41:39.471812+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.688455+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:09.478704+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-07T07:05:22.723382+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 4 -> "Wolfe's mineral elixir" @ 1 ml/l; 1 -> "Wolin's vitamin solution (10x)" @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |