stetteria_medium

CultureMech:001938 archaea COMPLEX LIQUID
Original name: STETTERIA MEDIUM

Overview

External ontology
mediadive.medium:795 (source link unavailable) — STETTERIA MEDIUM
Applications
  • Microbial cultivation
pH
6.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium795.pdf

Composition

flowchart LR
    CultureMech_001938["stetteria_medium"]
    ing0_NaHCO3["NaHCO3"]
    CultureMech_001938 --> ing0_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing0_NaHCO3 -.-> CHEBI_32139
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001938 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001938 --> ing2_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing2_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing3_Peptone["Peptone"]
    CultureMech_001938 --> ing3_Peptone
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_001938 --> ing4_Yeast_extract
    ing5_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001938 --> ing5_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Sodium_resazurin -.-> CHEBI_8806
    ing6_Sulfur["Sulfur"]
    CultureMech_001938 --> ing6_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing6_Sulfur -.-> CHEBI_26833
    ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001938 --> ing7_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_x_9_H2O -.-> CHEBI_76209
    ing8_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001938 --> ing8_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing8_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001938 --> ing9_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing10_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001938 --> ing10_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing10_MnSO4_x_H2O -.-> CHEBI_86364
    ing11_NaCl["NaCl"]
    CultureMech_001938 --> ing11_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing11_NaCl -.-> CHEBI_26710
    ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001938 --> ing12_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing13_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001938 --> ing13_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing13_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001938 --> ing14_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001938 --> ing15_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001938 --> ing16_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing16_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing17_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001938 --> ing17_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing17_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing18_H3BO3["H3BO3"]
    CultureMech_001938 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001938 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001938 --> ing20_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001938 --> ing21_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing22_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_001938 --> ing22_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing22_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing23_KI["KI"]
    CultureMech_001938 --> ing23_KI
    CHEBI_8346["CHEBI:8346"]
    ing23_KI -.-> CHEBI_8346
    ing24_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001938 --> ing24_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing24_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing25_Na3_citrate["Na3-citrate"]
    CultureMech_001938 --> ing25_Na3_citrate
    CHEBI_53258["CHEBI:53258"]
    ing25_Na3_citrate -.-> CHEBI_53258
    ing26_KCl["KCl"]
    CultureMech_001938 --> ing26_KCl
    CHEBI_32588["CHEBI:32588"]
    ing26_KCl -.-> CHEBI_32588
    ing27_NaBr["NaBr"]
    CultureMech_001938 --> ing27_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing27_NaBr -.-> CHEBI_63004

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 NaHCO3
CHO3.Na 84.006 g/mol
0.157171 G_PER_L CHEBI:32139
sodium hydrogencarbonate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.491159 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.032946949999999996 G_PER_L CHEBI:34887
nickel dichloride
 [Merged 2 duplicates: 0.00294695, 0.03]
4 Peptone 1.96464 G_PER_L
5 Yeast extract 0.982318 G_PER_L
6 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000491159 G_PER_L CHEBI:8806
Resazurin
7 Sulfur
S 32.067 g/mol
9.82318 G_PER_L CHEBI:26833
sulfur atom
 (powdered)
8 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.491159 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
9 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
10 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
17.0 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 3.0, 14.0]
11 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
12 NaCl
Cl.Na 58.443 g/mol
56.4 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 1.0, 55.4]
13 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
14 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
15 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.6 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.1, 1.5]
16 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
17 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
18 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
19 H3BO3
H3BO3 61.833 g/mol
0.06999999999999999 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.01, 0.06]
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
21 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
22 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
23 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.03 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
24 KI
I.K 166.002 g/mol
0.0001 G_PER_L CHEBI:8346
potassium iodide
25 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
11 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
26 Na3-citrate
C6H5O7.3Na 258.069 g/mol
0.02 G_PER_L CHEBI:53258
sodium citrate
27 KCl
Cl.K 74.551 g/mol
1.3 G_PER_L CHEBI:32588
potassium chloride
28 NaBr
Br.Na 102.894 g/mol
0.2 G_PER_L CHEBI:63004
sodium bromide

Preparation

  1. Dissolve ingredients except sulfur and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into serum vials (e.g., 20 ml medium per 100 ml bottle) that contain already the appropriate amount of sulfur. After closing pressurize bottles to 2 bar overpressure using 80% H2 and 20% CO2 gas mixture, than heat at 100°C for 1.5 hours on each of 3 successive days. Before use add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.0, if necessary. Incubate without shaking.
  2. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.280336Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 795
2026-03-13T03:08:45.446578Z data-quality-cleanup-v1.0 Data quality fixes Merged 5 duplicate ingredient(s)
2026-03-13T06:19:08.637992Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001938
2026-03-17T06:29:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.102789+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "STETTERIA MEDIUM" to "stetteria_medium" for consistent matching
2026-06-05T07:41:47.046872+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.855342+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.