Overview
- External ontology
- mediadive.medium:795a (source link unavailable) — LEBETIMONAS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium795a.pdf
Composition
flowchart LR
CultureMech_001939["lebetimonas_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_001939 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KH2PO4["KH2PO4"]
CultureMech_001939 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001939 --> ing2_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing2_NiCl2_x_6_H2O -.-> CHEBI_34887
ing3_Na_formate["Na-formate"]
CultureMech_001939 --> ing3_Na_formate
CHEBI_62965["CHEBI:62965"]
ing3_Na_formate -.-> CHEBI_62965
ing4_Tryptone["Tryptone"]
CultureMech_001939 --> ing4_Tryptone
ing5_Yeast_extract["Yeast extract"]
CultureMech_001939 --> ing5_Yeast_extract
ing6_Sodium_resazurin["Sodium resazurin"]
CultureMech_001939 --> ing6_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Sodium_resazurin -.-> CHEBI_8806
ing7_NaHCO3["NaHCO3"]
CultureMech_001939 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_Sulfur["Sulfur"]
CultureMech_001939 --> ing8_Sulfur
CHEBI_26833["CHEBI:26833"]
ing8_Sulfur -.-> CHEBI_26833
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001939 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_NaCl["NaCl"]
CultureMech_001939 --> ing10_NaCl
CHEBI_26710["CHEBI:26710"]
ing10_NaCl -.-> CHEBI_26710
ing11_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_001939 --> ing11_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing11_SrCl2_x_6_H2O -.-> CHEBI_36385
ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001939 --> ing12_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
ing13_KI["KI"]
CultureMech_001939 --> ing13_KI
CHEBI_8346["CHEBI:8346"]
ing13_KI -.-> CHEBI_8346
ing14_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001939 --> ing14_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing14_MgCl2_x_6_H2O -.-> CHEBI_86345
ing15_Na3_citrate["Na3-citrate"]
CultureMech_001939 --> ing15_Na3_citrate
CHEBI_53258["CHEBI:53258"]
ing15_Na3_citrate -.-> CHEBI_53258
ing16_KCl["KCl"]
CultureMech_001939 --> ing16_KCl
CHEBI_32588["CHEBI:32588"]
ing16_KCl -.-> CHEBI_32588
ing17_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001939 --> ing17_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing17_CaCl2_x_2_H2O -.-> CHEBI_86158
ing18_NaBr["NaBr"]
CultureMech_001939 --> ing18_NaBr
CHEBI_63004["CHEBI:63004"]
ing18_NaBr -.-> CHEBI_63004
ing19_H3BO3["H3BO3"]
CultureMech_001939 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001939 --> ing20_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing20_Nitrilotriacetic_acid -.-> CHEBI_44557
ing21_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001939 --> ing21_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing21_MnSO4_x_H2O -.-> CHEBI_86364
ing22_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001939 --> ing22_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing22_FeSO4_x_7_H2O -.-> CHEBI_75836
ing23_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001939 --> ing23_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing23_CoSO4_x_7_H2O -.-> CHEBI_91244
ing24_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001939 --> ing24_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing24_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing25_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001939 --> ing25_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing25_CuSO4_x_5_H2O -.-> CHEBI_31440
ing26_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001939 --> ing26_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing26_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing27_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001939 --> ing27_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing27_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing28_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001939 --> ing28_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing28_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing29_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001939 --> ing29_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing29_Na2WO4_x_2_H2O -.-> CHEBI_63939
CultureMech_001939 --> more["...10 more"]
Ingredients (40)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.23274 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.493097 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03295858 G_PER_L |
CHEBI:34887
nickel dichloride
|
[Merged 2 duplicates: 0.00295858, 0.03] |
| 4 |
Na-formate
CHO2.Na 68.007 g/mol
|
0.197239 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 5 | Tryptone | 0.986193 G_PER_L | ||
| 6 | Yeast extract | 0.986193 G_PER_L | ||
| 7 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000493097 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.97239 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
Sulfur
S 32.067 g/mol
|
9.86193 G_PER_L |
CHEBI:26833
sulfur atom
|
(powdered) |
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.493097 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
NaCl
Cl.Na 58.443 g/mol
|
56.4 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 55.4, 1.0] |
| 12 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.03 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 13 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
17.0 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 14.0, 3.0] |
| 14 |
KI
I.K 166.002 g/mol
|
0.0001 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 15 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
11 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 16 |
Na3-citrate
C6H5O7.3Na 258.069 g/mol
|
0.02 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 17 |
KCl
Cl.K 74.551 g/mol
|
1.3 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 18 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 1.5, 0.1] |
| 19 |
NaBr
Br.Na 102.894 g/mol
|
0.2 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.06999999999999999 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.06, 0.01] |
| 21 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 22 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 23 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 24 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 25 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 26 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 27 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 28 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 29 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 30 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 31 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 32 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 33 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 34 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 35 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 36 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 37 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 38 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 39 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 40 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients (except bicarbonate, vitamins, sulfur, and sulfide), then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic and adjust pH to 5.5 with H2SO4. Dispense under the same gas atmosphere in suitable culture vessels (e.g. 20 ml of the medium in 100 ml serum bottles) and autoclave. Steam sulfur for 3 hours on each of 3 successive days (see medium 35). Aseptically mix the sterilized sulfur with the sterile medium while retaining anoxic conditions. Add vitamins from an anoxic stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to inoculation add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere. Check pH and adjust to 5.5, if necessary.
- After inoculation pressurize vials to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.285014Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 795a |
| 2026-02-06T05:10:25.019677Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:08:57.841112Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:19:08.654872Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001939 |
| 2026-03-17T06:30:02.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:21.121106+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LEBETIMONAS MEDIUM" to "lebetimonas_medium" for consistent matching |
| 2026-06-05T07:41:11.026165+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 35 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.513976+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |