modified_chopped_meat_medium

Overview

External ontology

mediadive.medium:797 (source link unavailable) — MODIFIED CHOPPED MEAT MEDIUM

Applications

• Microbial cultivation

pH

7.0

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium797.pdf

Composition

flowchart LR
    CultureMech_001941["modified_chopped_meat_medium"]
    ing0_Ground_beef["Ground beef"]
    CultureMech_001941 --> ing0_Ground_beef
    ing1_NaOH["NaOH"]
    CultureMech_001941 --> ing1_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing1_NaOH -.-> CHEBI_32145
    ing2_Trypticase["Trypticase"]
    CultureMech_001941 --> ing2_Trypticase
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_001941 --> ing3_Yeast_extract
    ing4_K2HPO4["K2HPO4"]
    CultureMech_001941 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Resazurin["Resazurin"]
    CultureMech_001941 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_Agar["Agar"]
    CultureMech_001941 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001941 --> ing7_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing8_Vitamin_K1["Vitamin K1"]
    CultureMech_001941 --> ing8_Vitamin_K1
    CHEBI_18067["CHEBI:18067"]
    ing8_Vitamin_K1 -.-> CHEBI_18067
    ing9_Ethanol["Ethanol"]
    CultureMech_001941 --> ing9_Ethanol
    CHEBI_16236["CHEBI:16236"]
    ing9_Ethanol -.-> CHEBI_16236
    ing10_Haemin["Haemin"]
    CultureMech_001941 --> ing10_Haemin
    CHEBI_50385["CHEBI:50385"]
    ing10_Haemin -.-> CHEBI_50385

Ingredients (11)

Preparation

1. Mix meat, water and NaOH, and bring to a boil with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add sufficient distilled water to restore volume to 1000 ml. To this filtrate add:
2. Boil and cool medium under 80% N2, 10% H2, 10% CO2, and add:
3. Adjust medium for final pH 7.0. Anaerobically dispense 7 ml into tubes containing meat particles (use 1 part meat to 5 parts fluid) under the same gas phase.
4. Store solution in brown bottle under refrigeration. Discard after one month.
5. Autoclave at 121°C for 15 min.

Target organisms

No target organisms are recorded.

Provenance