sphaerotilus_leptothrix_medium (CultureMech:001947)

Overview

External ontology

mediadive.medium:803 (source link unavailable) — SPHAEROTILUS-LEPTOTHRIX MEDIUM

Applications

Microbial cultivation

pH

7.1

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium803.pdf

Composition

flowchart LR
    CultureMech_001947["sphaerotilus_leptothrix_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001947 --> ing0_Yeast_extract
    ing1_Peptone["Peptone"]
    CultureMech_001947 --> ing1_Peptone
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001947 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2["CaCl2"]
    CultureMech_001947 --> ing3_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing3_CaCl2 -.-> CHEBI_3312
    ing4_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_001947 --> ing4_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing4_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing5_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001947 --> ing5_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing5_MnSO4_x_H2O -.-> CHEBI_86364
    ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_001947 --> ing6_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing7_Agar["Agar"]
    CultureMech_001947 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Tap_water["Tap water"]
    CultureMech_001947 --> ing8_Tap_water
    CHEBI_15377["CHEBI:15377"]
    ing8_Tap_water -.-> CHEBI_15377

Ingredients (9)

#	Ingredient	Concentration	Ontology	Notes
1	Yeast extract	1 G_PER_L
2	Peptone	1.5 G_PER_L
3	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.2 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
4	CaCl2 Ca.2Cl 110.984 g/mol	0.05 G_PER_L	CHEBI:3312 calcium dichloride
5	Ferric ammonium citrate	0.5 G_PER_L	CHEBI:31604 ferric ammonium citrate
6	MnSO4 x H2O H2O.Mn.O4S 169.016 g/mol	0.05 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate
7	FeCl3 x 6 H2O Cl3Fe.6H2O 270.294 g/mol	0.01 G_PER_L	CHEBI:86254 iron trichloride hexahydrate
8	Agar	20 G_PER_L	CHEBI:2509 agar
9	Tap water H2O 18.015 g/mol	1 L	CHEBI:15377 water	Source batch input: 1000 ml tap water = 1 L. This is an input volume, not a mass concentration, volume percentage or measured final-medium volume. No density or final-volume assumption is made.

Preparation

pH 7.1.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:12.306740Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 803
2026-03-13T06:19:08.719900Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:001947
2026-03-17T06:30:48.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.191146+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "SPHAEROTILUS-LEPTOTHRIX MEDIUM" to "sphaerotilus_leptothrix_medium" for consistent matching
2026-06-05T07:41:46.157306+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-07T12:21:30.028294+00:00	codex	CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.
2026-10-08T07:20:58.579071+00:00	codex	CORRECTED_DSMZ803_1046_1065_WATER_INPUT_UNITS	Original DSMZ PDF and exact linked API recipe independently inspected. Preserved prior evidence/history, identifiers, non-water amounts, preparation, chemical mappings and parent/variant links. No final-volume, density, stock dilution or growth inference.