Overview
- External ontology
- mediadive.medium:804 (source link unavailable) — HAEMOPHILUS MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium804.pdf Commercial Product: Mueller-Hinton Agar Supplier: Multiple suppliers (BD, Sigma-Aldrich, HiMedia) Catalog: BD 211443, Sigma 70191 Source: https://microbenotes.com/mueller-hinton-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_001948["haemophilus_medium"]
ing0_Beef_extract["Beef extract"]
CultureMech_001948 --> ing0_Beef_extract
ing1_Acid_hydrolysate_of_casein["Acid hydrolysate of casein"]
CultureMech_001948 --> ing1_Acid_hydrolysate_of_casein
ing2_Starch["Starch"]
CultureMech_001948 --> ing2_Starch
CHEBI_28017["CHEBI:28017"]
ing2_Starch -.-> CHEBI_28017
ing3_Agar["Agar"]
CultureMech_001948 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_Yeast_extract["Yeast extract"]
CultureMech_001948 --> ing4_Yeast_extract
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Beef extract
Multiple suppliers (BD, Sigma-Aldrich, HiMedia) Mueller-Hinton Agar BD 211443, Sigma 70191
|
2.0 G_PER_L |
FOODON:03302088
beef extract
|
Aqueous beef extract providing nutrients and growth factors; Constituent of Mueller-Hinton Agar commercial product |
| 2 |
Acid hydrolysate of casein
Multiple suppliers (BD, Sigma-Aldrich, HiMedia) Mueller-Hinton Agar BD 211443, Sigma 70191
|
17.5 G_PER_L |
MICRO:0001366 (source link unavailable)
casein hydrolysate
|
Casein enzymatic hydrolysate, provides amino acids and peptides. Low PABA and thymine content critical for sulfonamide/trimethoprim testing; Constituent of Mueller-Hinton Agar commercial product |
| 3 |
Starch
Multiple suppliers (BD, Sigma-Aldrich, HiMedia) Mueller-Hinton Agar BD 211443, Sigma 70191
|
1.5 G_PER_L |
CHEBI:28017
starch
|
Acts as protective colloid and absorbs toxic metabolites released from bacteria; Constituent of Mueller-Hinton Agar commercial product |
| 4 |
Agar
Multiple suppliers (BD, Sigma-Aldrich, HiMedia) Mueller-Hinton Agar BD 211443, Sigma 70191
|
17.0 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent; Constituent of Mueller-Hinton Agar commercial product |
| 5 | Yeast extract | 5 G_PER_L |
Preparation
- For Haemophilus spp., add 15 mg NAD and 15 mg hemine per L (X + V factors).
- NAD stock solution: dissolve 1 mg NAD per ml dist. water, filter sterilize.
- Hemine stock solution: dissolve 50 mg hemine in 1 ml 1 M NaOH, make up to 25 ml with dist. water, filter sterilize.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.308368Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 804 |
| 2026-03-13T06:19:08.723221Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001948 |
| 2026-03-17T19:45:21.194707+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HAEMOPHILUS MEDIUM" to "haemophilus_medium" for consistent matching |
| 2026-05-17T07:06:44.776757+00:00 | cleanup_residual_errors.py | CLEANUP_RESIDUAL_ERRORS | R01_flatten_synonym_text=2 |