methylobacterium_thiocyanatum_medium

CultureMech:001949 bacterial DEFINED SOLID_AGAR
Original name: METHYLOBACTERIUM THIOCYANATUM MEDIUM

Overview

External ontology
mediadive.medium:805 (source link unavailable) — METHYLOBACTERIUM THIOCYANATUM MEDIUM
Applications
  • Microbial cultivation
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium805.pdf

Composition

flowchart LR
    CultureMech_001949["methylobacterium_thiocyanatum_medium"]
    ing0_KSCN["KSCN"]
    CultureMech_001949 --> ing0_KSCN
    CHEBI_30951["CHEBI:30951"]
    ing0_KSCN -.-> CHEBI_30951
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001949 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_K2HPO4["K2HPO4"]
    CultureMech_001949 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_001949 --> ing3_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing3_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing4_Glucose["Glucose"]
    CultureMech_001949 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001949 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing6_Agar["Agar"]
    CultureMech_001949 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 KSCN
CNS.K 97.183 g/mol
0.25 G_PER_L CHEBI:30951
potassium thiocyanate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 K2HPO4
HO4P.2K 174.174 g/mol
1.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
7.9 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
5 Glucose
C6H12O6 180.156 g/mol
4.5 G_PER_L CHEBI:17234
glucose
6 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.02 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
7 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)

Preparation

  1. To solid media add 15 g/l agar. Sterilise medium at 115°C for 10 minutes.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.309458Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 805
2026-03-13T06:19:08.727016Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001949
2026-03-17T06:30:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.198831+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOBACTERIUM THIOCYANATUM MEDIUM" to "methylobacterium_thiocyanatum_medium" for consistent matching
2026-06-05T07:41:33.028533+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.520930+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T12:21:30.028294+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.