haloanaerobium_alcaliphilum_medium

CultureMech:001951 bacterial COMPLEX LIQUID
Original name: HALOANAEROBIUM ALCALIPHILUM MEDIUM

Overview

External ontology
mediadive.medium:807 (source link unavailable) — HALOANAEROBIUM ALCALIPHILUM MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium807.pdf

Composition

flowchart LR
    CultureMech_001951["haloanaerobium_alcaliphilum_medium"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001951 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_NaCl["NaCl"]
    CultureMech_001951 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_Resazurin["Resazurin"]
    CultureMech_001951 --> ing2_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing2_Resazurin -.-> CHEBI_8806
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_001951 --> ing3_Yeast_extract
    ing4_Trypticase["Trypticase"]
    CultureMech_001951 --> ing4_Trypticase
    ing5_Glucose["Glucose"]
    CultureMech_001951 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001951 --> ing6_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing6_Na2S_x_9_H2O -.-> CHEBI_76209
    ing7_NaHCO3["NaHCO3"]
    CultureMech_001951 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001951 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_K2HPO4["K2HPO4"]
    CultureMech_001951 --> ing9_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing9_K2HPO4 -.-> CHEBI_131527
    ing10_KH2PO4["KH2PO4"]
    CultureMech_001951 --> ing10_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing10_KH2PO4 -.-> CHEBI_63036
    ing11__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001951 --> ing11__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing11__NH4_2SO4 -.-> CHEBI_62946
    ing12_NH4Cl["NH4Cl"]
    CultureMech_001951 --> ing12_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing12_NH4Cl -.-> CHEBI_31206
    ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001951 --> ing13_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing14_HCl["HCl"]
    CultureMech_001951 --> ing14_HCl
    CHEBI_17883["CHEBI:17883"]
    ing14_HCl -.-> CHEBI_17883
    ing15_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001951 --> ing15_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing15_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing16_ZnCl2["ZnCl2"]
    CultureMech_001951 --> ing16_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing16_ZnCl2 -.-> CHEBI_49976
    ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001951 --> ing17_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing18_H3BO3["H3BO3"]
    CultureMech_001951 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001951 --> ing19_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001951 --> ing20_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001951 --> ing21_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001951 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
19.6 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 17.0, 2.6]
2 NaCl
Cl.Na 58.443 g/mol
112.0 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 100.0, 12.0]
3 Resazurin
C12H7NO4 229.191 g/mol
0.001 G_PER_L CHEBI:8806
Resazurin
4 Yeast extract 10 G_PER_L
5 Trypticase 10 G_PER_L MICRO:0000175 (source link unavailable)
Trypticase peptone
6 Glucose
C6H12O6 180.156 g/mol
5 G_PER_L CHEBI:17234
glucose
7 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.25 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
8 NaHCO3
CHO3.Na 84.006 g/mol
4.1 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
10 K2HPO4
HO4P.2K 174.174 g/mol
6.28 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 [Merged 2 duplicates: 6.0, 0.28]
11 KH2PO4
H2O4P.K 136.084 g/mol
6 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
12 (NH4)2SO4
2H4N.O4S 132.141 g/mol
6 G_PER_L CHEBI:62946
ammonium sulfate
13 NH4Cl
Cl.H4N 53.492 g/mol
2.5 G_PER_L CHEBI:31206
ammonium chloride
14 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.28 G_PER_L CHEBI:86158
calcium chloride dihydrate
15 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
16 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
17 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
18 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
19 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
20 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
21 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
22 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. pH 7.0
  2. Prepare the medium without yeast extract, glucose, NaHCO3, cysteine hydrochloride and Na2S x 9 H2O. Boil and then cool under a gas atmosphere of N2:CO2 (80:20 v/v) and add the NaHCO3 and cysteine hydrochloride. Autoclave the medium and add from sterile stock solutions yeast extract, trypticase, glucose, and Na2S x 9 H2O. The final pH should be 7.0.
  3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.316134Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 807
2026-01-28T19:48:40.930349Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:40.630217Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:08.758045Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001951
2026-03-17T06:30:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.227075+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOANAEROBIUM ALCALIPHILUM MEDIUM" to "haloanaerobium_alcaliphilum_medium" for consistent matching
2026-06-05T07:41:07.393480+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.400306+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-22T00:03:26.686152+00:00 repair_rejected_peptone_groundings.py CORRECTED_REJECTED_PEPTONE_GROUNDING CultureMech #484. /ingredients/4/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved.