glycerol_soil_medium

Overview

External ontology

mediadive.medium:80 (source link unavailable) — GLYCEROL-SOIL MEDIUM

Applications

• Microbial cultivation

pH

7.0

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium80.pdf

Composition

flowchart LR
    CultureMech_001954["glycerol_soil_medium"]
    ing0_Peptone["Peptone"]
    CultureMech_001954 --> ing0_Peptone
    ing1_Beef_extract["Beef extract"]
    CultureMech_001954 --> ing1_Beef_extract
    ing2_Glycerol["Glycerol"]
    CultureMech_001954 --> ing2_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing2_Glycerol -.-> CHEBI_17754
    ing3_Soil_extract["Soil extract"]
    CultureMech_001954 --> ing3_Soil_extract
    ing4_Agar["Agar"]
    CultureMech_001954 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509

Ingredients (5)

Preparation

1. Adjust pH to 7.0. Soil extract is prepared by sieving air dried garden soil through a coarse sieve and autoclaving 400 g with 960 ml of distilled water at 121°C for one hour. After the mixture is cool and settled, the supernatant is carefully decanted, filtered through paper, autoclaved in 200 ml quantities, and stored at room temperature until cleared by sedimentation.

Target organisms

No target organisms are recorded.

Provenance