sulfobacillus_disulfidooxidans_medium

CultureMech:001957 bacterial COMPLEX LIQUID
Original name: SULFOBACILLUS DISULFIDOOXIDANS MEDIUM

Overview

External ontology
mediadive.medium:812 (source link unavailable) — SULFOBACILLUS DISULFIDOOXIDANS MEDIUM
Applications
  • Microbial cultivation
pH
2.3
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium812.pdf

Composition

flowchart LR
    CultureMech_001957["sulfobacillus_disulfidooxidans_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001957 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KCl["KCl"]
    CultureMech_001957 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001957 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001957 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_001957 --> ing4_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing4_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_001957 --> ing5_Yeast_extract
    ing6_Glutathione["Glutathione"]
    CultureMech_001957 --> ing6_Glutathione
    CHEBI_16856["CHEBI:16856"]
    ing6_Glutathione -.-> CHEBI_16856

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
3 G_PER_L CHEBI:62946
ammonium sulfate
2 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
3 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
0.1 G_PER_L CHEBI:86159
calcium nitrate tetrahydrate
6 Yeast extract 0.1 G_PER_L
7 Glutathione
C10H17N3O6S 307.328 g/mol
1 G_PER_L CHEBI:16856
glutathione

Preparation

  1. Adjust pH to 2.25 prior to autoclaving. Before use add per liter 10 ml of a filter sterilized 10% (w/v) glutathione solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.328028Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 812
2026-03-13T06:19:08.793066Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001957
2026-03-17T06:30:23.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.268775+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SULFOBACILLUS DISULFIDOOXIDANS MEDIUM" to "sulfobacillus_disulfidooxidans_medium" for consistent matching
2026-06-05T07:41:47.375265+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.988984+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T12:21:30.028294+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.