Overview
- External ontology
- mediadive.medium:814 (source link unavailable) — METHANOBACTERIUM SUBTERRANEUM MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 8.0, 'max': 8.5}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium814.pdf
Composition
flowchart LR
CultureMech_001961["methanobacterium_subterraneum_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_001961 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001961 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_NaCl["NaCl"]
CultureMech_001961 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_001961 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001961 --> ing4_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
ing5_Tryptone["Tryptone"]
CultureMech_001961 --> ing5_Tryptone
ing6_Na_formate["Na-formate"]
CultureMech_001961 --> ing6_Na_formate
CHEBI_62965["CHEBI:62965"]
ing6_Na_formate -.-> CHEBI_62965
ing7_Na_acetate["Na-acetate"]
CultureMech_001961 --> ing7_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing7_Na_acetate -.-> CHEBI_32954
ing8_Sodium_resazurin["Sodium resazurin"]
CultureMech_001961 --> ing8_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Sodium_resazurin -.-> CHEBI_8806
ing9_Na2CO3["Na2CO3"]
CultureMech_001961 --> ing9_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing9_Na2CO3 -.-> CHEBI_29377
ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001961 --> ing10_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001961 --> ing11_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing11_Na2S_x_9_H2O -.-> CHEBI_76209
ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001961 --> ing12_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001961 --> ing13_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
ing14_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001961 --> ing14_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing14_MnSO4_x_H2O -.-> CHEBI_86364
ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001961 --> ing15_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
ing16_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001961 --> ing16_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing16_CaCl2_x_2_H2O -.-> CHEBI_86158
ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001961 --> ing17_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001961 --> ing18_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
ing19_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001961 --> ing19_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing19_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing20_H3BO3["H3BO3"]
CultureMech_001961 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001961 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001961 --> ing22_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001961 --> ing23_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001961 --> ing24_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.394867 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.029615 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
1.4442249999999999 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 0.444225, 1.0] |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.493583 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.10296150000000001 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.0029615, 0.1] |
| 6 | Tryptone | 0.987167 G_PER_L | ||
| 7 |
Na-formate
CHO2.Na 68.007 g/mol
|
3.15893 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 8 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.987167 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 9 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000493583 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.987167 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 11 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.246792 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 12 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.246792 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 13 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 14 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 15 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 16 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 17 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 18 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 19 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 20 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 24 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 25 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve ingredients except carbonate, vitamins, cysteine and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 8.0 - 8.5.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.342534Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 814 |
| 2026-03-13T03:09:20.855266Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:08.844314Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001961 |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:21.328561+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOBACTERIUM SUBTERRANEUM MEDIUM" to "methanobacterium_subterraneum_medium" for consistent matching |
| 2026-05-20T06:01:55.143948+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '8-8.5' -> {'min': 8.0, 'max': 8.5} |
| 2026-06-05T07:41:30.821124+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.704808+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |