Overview
- External ontology
- mediadive.medium:817 (source link unavailable) — THERMOSPHAERA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium817.pdf
Composition
flowchart LR
CultureMech_001964["thermosphaera_medium"]
ing0_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001964 --> ing0_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing0_MgCl2_x_6_H2O -.-> CHEBI_86345
ing1_NaCl["NaCl"]
CultureMech_001964 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_KCl["KCl"]
CultureMech_001964 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_NH4Cl["NH4Cl"]
CultureMech_001964 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001964 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_001964 --> ing5_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing6_FeCl3["FeCl3"]
CultureMech_001964 --> ing6_FeCl3
CHEBI_30808["CHEBI:30808"]
ing6_FeCl3 -.-> CHEBI_30808
ing7_Yeast_extract["Yeast extract"]
CultureMech_001964 --> ing7_Yeast_extract
ing8_Peptone["Peptone"]
CultureMech_001964 --> ing8_Peptone
ing9_Sodium_resazurin["Sodium resazurin"]
CultureMech_001964 --> ing9_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Sodium_resazurin -.-> CHEBI_8806
ing10_Na2CO3["Na2CO3"]
CultureMech_001964 --> ing10_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing10_Na2CO3 -.-> CHEBI_29377
ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001964 --> ing11_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing11_Na2S_x_9_H2O -.-> CHEBI_76209
ing12_Biotin["Biotin"]
CultureMech_001964 --> ing12_Biotin
CHEBI_15956["CHEBI:15956"]
ing12_Biotin -.-> CHEBI_15956
ing13_Folic_acid["Folic acid"]
CultureMech_001964 --> ing13_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing13_Folic_acid -.-> CHEBI_27470
ing14_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001964 --> ing14_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing14_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing15_Thiamine_HCl["Thiamine HCl"]
CultureMech_001964 --> ing15_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing15_Thiamine_HCl -.-> CHEBI_49105
ing16_Riboflavin["Riboflavin"]
CultureMech_001964 --> ing16_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing16_Riboflavin -.-> CHEBI_17015
ing17_Nicotinic_acid["Nicotinic acid"]
CultureMech_001964 --> ing17_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing17_Nicotinic_acid -.-> CHEBI_15940
ing18_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001964 --> ing18_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing18_Calcium_D_____pantothenate -.-> CHEBI_31345
ing19_Vitamin_B12["Vitamin B12"]
CultureMech_001964 --> ing19_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing19_Vitamin_B12 -.-> CHEBI_176843
ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001964 --> ing20_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
ing21__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_001964 --> ing21__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing21__DL__alpha_Lipoic_acid -.-> CHEBI_16494
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.16749 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
0.886699 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.0167488 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.0118227 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.00689655 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
0.00689655 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
FeCl3
Cl3Fe 162.204 g/mol
|
4.92611e-05 G_PER_L |
CHEBI:30808
iron trichloride
|
|
| 8 | Yeast extract | 0.985222 G_PER_L | ||
| 9 | Peptone | 0.985222 G_PER_L | ||
| 10 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000492611 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.492611 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 12 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.492611 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 13 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 14 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 15 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 16 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 17 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 18 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 19 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 20 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 21 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 22 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients except carbonate, vitamins and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the complete medium should be adjusted to 6.5.
- After inoculation pressurize tubes or bottles (heavy walled) to 2 bar overpressure with sterile 80% N2 and 20% CO2 gas mixture.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.356770Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 817 |
| 2026-02-06T05:10:44.062230Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:08.878414Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001964 |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:21.365855+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "THERMOSPHAERA MEDIUM" to "thermosphaera_medium" for consistent matching |
| 2026-06-05T07:41:50.590284+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:13.184393+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |