Overview
- External ontology
- mediadive.medium:826 (source link unavailable) — GEOBACTER SULFURREDUCENS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium826.pdf
Composition
flowchart LR
CultureMech_001975["geobacter_sulfurreducens_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_001975 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_Na2HPO4["Na2HPO4"]
CultureMech_001975 --> ing1_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing1_Na2HPO4 -.-> CHEBI_34683
ing2_KCl["KCl"]
CultureMech_001975 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_Na_acetate["Na-acetate"]
CultureMech_001975 --> ing3_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing3_Na_acetate -.-> CHEBI_32954
ing4_NaHCO3["NaHCO3"]
CultureMech_001975 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_Na2_fumarate["Na2-fumarate"]
CultureMech_001975 --> ing5_Na2_fumarate
CHEBI_115156["CHEBI:115156"]
ing5_Na2_fumarate -.-> CHEBI_115156
ing6_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001975 --> ing6_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing6_Nitrilotriacetic_acid -.-> CHEBI_44557
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001975 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001975 --> ing8_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing8_MnSO4_x_H2O -.-> CHEBI_86364
ing9_NaCl["NaCl"]
CultureMech_001975 --> ing9_NaCl
CHEBI_26710["CHEBI:26710"]
ing9_NaCl -.-> CHEBI_26710
ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001975 --> ing10_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
ing11_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001975 --> ing11_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing11_CoSO4_x_7_H2O -.-> CHEBI_91244
ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001975 --> ing12_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_x_2_H2O -.-> CHEBI_86158
ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001975 --> ing13_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing14_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001975 --> ing14_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing14_CuSO4_x_5_H2O -.-> CHEBI_31440
ing15_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001975 --> ing15_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing15_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing16_H3BO3["H3BO3"]
CultureMech_001975 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001975 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001975 --> ing18_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001975 --> ing19_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing19_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing20_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001975 --> ing20_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing20_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing21_Biotin["Biotin"]
CultureMech_001975 --> ing21_Biotin
CHEBI_15956["CHEBI:15956"]
ing21_Biotin -.-> CHEBI_15956
ing22_Folic_acid["Folic acid"]
CultureMech_001975 --> ing22_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing22_Folic_acid -.-> CHEBI_27470
ing23_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001975 --> ing23_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing23_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing24_Thiamine_HCl["Thiamine HCl"]
CultureMech_001975 --> ing24_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing24_Thiamine_HCl -.-> CHEBI_49105
ing25_Riboflavin["Riboflavin"]
CultureMech_001975 --> ing25_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing25_Riboflavin -.-> CHEBI_17015
ing26_Nicotinic_acid["Nicotinic acid"]
CultureMech_001975 --> ing26_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing26_Nicotinic_acid -.-> CHEBI_15940
ing27_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001975 --> ing27_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing27_Calcium_D_____pantothenate -.-> CHEBI_31345
ing28_Vitamin_B12["Vitamin B12"]
CultureMech_001975 --> ing28_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing28_Vitamin_B12 -.-> CHEBI_176843
ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001975 --> ing29_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
CultureMech_001975 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.48368 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.593472 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.098912 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.811078 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.4728 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 6 |
Na2-fumarate
C4H2O4.2Na 160.036 g/mol
|
7.91296 G_PER_L |
CHEBI:115156
disodium fumarate
|
|
| 7 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 11 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 12 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 13 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 14 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 15 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 16 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 20 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 21 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 22 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 23 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 24 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 25 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 26 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 27 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 28 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 29 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 30 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 31 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients (except bicarbonate, fumarate and vitamins), sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add bicarbonate and equilibrate the medium with 80% N2 and 20% CO2 gas mixture to reach a pH of 6.8, then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add fumarate and vitamins from anoxic stock solutions prepared under 100% N2 gas atmosphere and sterilized by filtration. Check medium pH and adjust to 6.8, if necessary.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms and growth evidence
These are curated observations and conditions for named organisms; they do not imply universal growth suitability.
- Preferred term
- Geobacter sulfurreducens
- Term
- Id
- NCBITaxon:35554
- Label
- Geobacter sulfurreducens
- Growth metrics
- Doubling time minutes
- 300.0
- Evidence
- Reference
- PMID:35960254
- Supports
- SUPPORT
- Snippet
- engineering of the slow-growing Geobacter sulfurreducens (a doubling time of 5 h with acetate as the electron donor and fumarate as the electron acceptor)
- Explanation
- Auto-applied from literature query: targeted: Geobacter sulfurreducens doubling time
- Is max attainment
- True
Recipe variants
- Name
- acetate_fumarate_geobacter_sulfurreducens_growth
- Description
- Geobacter sulfurreducens growth with acetate as electron donor and fumarate as electron acceptor.
- Modifications
- Use acetate as the electron donor.
- Use fumarate as the electron acceptor.
- Use Geobacter sulfurreducens as the target organism.
- Purpose
- Capture electron donor/acceptor-specific growth evidence under the parent Geobacter sulfurreducens Medium record.
- Evidence
- Reference
- PMID:35960254
- Supports
- SUPPORT
- Snippet
- engineering of the slow-growing Geobacter sulfurreducens (a doubling time of 5 h with acetate as the electron donor and fumarate as the electron acceptor)
- Explanation
- Literature evidence supports modeling the acetate/fumarate growth condition under the parent Geobacter sulfurreducens Medium record.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.389425Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 826 |
| 2026-02-06T05:10:31.346117Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:09.004914Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001975 |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:21.510989+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GEOBACTER SULFURREDUCENS MEDIUM" to "geobacter_sulfurreducens_medium" for consistent matching |
| 2026-05-04T02:34:24.019323+00:00 | literature_review_apply | ADDED_GROWTH_EVIDENCE | organisms_added=1 metrics_added=1 genomes_added=0 |
| 2026-06-05T07:41:06.177106+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.032989+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T00:58:02.743865+00:00 | curate_organism_culture_type.py | SET_ORGANISM_CULTURE_TYPE | Inferred isolate: 1 specific strain(s) named, no community signal (#142) |