nevskia_medium

CultureMech:001977 bacterial DEFINED SOLID_AGAR
Original name: NEVSKIA MEDIUM

Overview

External ontology
mediadive.medium:828 (source link unavailable) — NEVSKIA MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 7.2}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium828.pdf

Composition

flowchart LR
    CultureMech_001977["nevskia_medium"]
    ing0_Potassium_phosphate_buffer["Potassium phosphate buffer"]
    CultureMech_001977 --> ing0_Potassium_phosphate_buffer
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001977 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001977 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_Na_lactate["Na-lactate"]
    CultureMech_001977 --> ing3_Na_lactate
    CHEBI_75228["CHEBI:75228"]
    ing3_Na_lactate -.-> CHEBI_75228
    ing4_Agar["Agar"]
    CultureMech_001977 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509
    ing5_HCl["HCl"]
    CultureMech_001977 --> ing5_HCl
    CHEBI_17883["CHEBI:17883"]
    ing5_HCl -.-> CHEBI_17883
    ing6_ZnCl2["ZnCl2"]
    CultureMech_001977 --> ing6_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing6_ZnCl2 -.-> CHEBI_49976
    ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001977 --> ing7_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing8_H3BO3["H3BO3"]
    CultureMech_001977 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001977 --> ing9_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing10_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001977 --> ing10_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing10_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001977 --> ing11_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001977 --> ing12_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001977 --> ing13_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing14_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001977 --> ing14_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing14_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing15_D_____biotin["D-(+)-biotin"]
    CultureMech_001977 --> ing15_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing15_D_____biotin -.-> CHEBI_15956
    ing16_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001977 --> ing16_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing16_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Potassium phosphate buffer 64.35 G_PER_L (1M, pH 7)
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.05 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.014 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 Na-lactate
C3H5O3.Na 112.06 g/mol
0.56 G_PER_L CHEBI:75228
sodium lactate
5 Agar 15 G_PER_L CHEBI:2509
agar
 (if necessary)
6 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
7 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
8 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
9 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
10 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
11 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
12 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
13 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
14 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
12.8 G_PER_L CHEBI:44557
nitrilotriacetic acid
15 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.08 G_PER_L CHEBI:30753
4-aminobenzoic acid
16 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
17 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate

Preparation

  1. Adjust the pH to 7.0 - 7.2.
  2. Filter sterilize the vitamin solution and add to the autoclaved medium.
  3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
  4. Adjust pH to 6.0 .

Solutions

SL-9

Dose: 0.5 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeCl2 x 4 H2O 2 G_PER_L

Seven vitamins solution

Dose: 0.5 ML_PER_L

Stock composition
IngredientConcentrationNotes
Vitamin B12 0.1 G_PER_L
Nicotinic acid 0.2 G_PER_L
Pyridoxine hydrochloride 0.3 G_PER_L
Thiamine-HCl x 2 H2O 0.2 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.397944Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 828
2026-03-13T06:19:09.030259Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001977
2026-03-17T06:29:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.539619+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NEVSKIA MEDIUM" to "nevskia_medium" for consistent matching
2026-05-20T06:02:00.975685+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2}
2026-06-05T07:41:39.629738+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.536179+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.748077+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 1 -> 'SL-9' @ 0.5 ml/l; 4 -> 'Seven vitamins solution' @ 0.5 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).