desulfurobacterium_medium

Overview

External ontology

mediadive.medium:829 (source link unavailable) — DESULFUROBACTERIUM MEDIUM

Applications

• Microbial cultivation

pH

6.0

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium829.pdf Superseded historical provenance, not a current formulation claim: Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The original reports final volume 1005 ml, while its listed main liquid inputs sum to 1005.5 ml. Preserve the discrepancy. Source-reported basal rates do not certify independently measured final volume; no missing makeup water is invented. Full exact-source recipe and stock/preparation tables were inspected. Reconstructed nodes retain explicit source scope; complete prior ingredient/stock assertions, including copied LB mappings and stage conflations, are archived in append-only history. A title alone is not the basis for removal. Unchanged annotations are preserved, not recertified; no new organism-growth association is added. Full exact-source recipe and stock/preparation tables were inspected. Reconstructed nodes retain explicit source scope; complete prior ingredient/stock assertions, including copied LB mappings and stage conflations, are archived in append-only history. A title alone is not the basis for removal. Unchanged annotations are preserved, not recertified; no new organism-growth association is added.

Composition

flowchart LR
    CultureMech_001978["desulfurobacterium_medium"]
    ing0_Sea_Salt["Sea Salt"]
    CultureMech_001978 --> ing0_Sea_Salt
    ing1_NH4Cl["NH4Cl"]
    CultureMech_001978 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001978 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_MES__2__N_morpholino__ethane_sulfonic_acid_["MES [2-(N-morpholino) ethane sulfonic acid]"]
    CultureMech_001978 --> ing3_MES__2__N_morpholino__ethane_sulfonic_acid_
    CHEBI_39005["CHEBI:39005"]
    ing3_MES__2__N_morpholino__ethane_sulfonic_acid_ -.-> CHEBI_39005
    ing4_Sulfur["Sulfur"]
    CultureMech_001978 --> ing4_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing4_Sulfur -.-> CHEBI_26833
    ing5_Na2CO3["Na2CO3"]
    CultureMech_001978 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_Distilled_water["Distilled water"]
    CultureMech_001978 --> ing6_Distilled_water

Ingredients (7)

Preparation

1. Dissolve ingredients (except sulfur, carbonate, vitamins, growth-stimulating factors and Na-dithionite), boil medium for 1 min, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere. Adjust pH to 6.0 and dispense under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials which contain already the appropriate amount of sulfur, only to 30% of their volume to allow for a large head space. Autoclave at a temperature of <b>110°C</b> for 20 min! Add vitamins and growth-stimulating factors from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate and Na-dithionite from sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins and Na-dithionite are sterilized by filtration. Adjust pH of the complete medium to 6.0, if necessary.
2. After inoculation use 1 bar overpressure of sterile 80% H2 and 20% CO2 gas mixture.

Solutions

Target organisms

No target organisms are recorded.

Recipe variants

• Name

  DSM 16661 and DSM 21157 source prescription

  Relationship

  STRAIN_SPECIFIC_VARIANT

  Modifications

  • Omit growth-stimulating factors and pressurize vials after inoculation to 2 bar with sterile 80% H2 and 20% CO2 gas mixture.

  Evidence

  • Reference

    https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium829.pdf

    Supports

    SUPPORT

    Snippet

    For DSM 16661, DSM 21157: Omit growth-stimulating factors and pressurize vials after inoculation to 2 bar with sterile 80% H2 and 20% CO2 gas mixture.

    Explanation

    variants[0].name: Original strain-specific instruction, separate from the base recipe; no measured growth association is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T03:35:49.362734+00:00; capture SHA256=8441671f114af61c997ae71ee4bf7c9e200d1fdf45764fe120e6a0709b35df80. Additional exact excerpt: For DSM 16661, DSM 21157: Omit growth-stimulating factors and pressurize vials after inoculation to 2 bar with sterile 80% H2 and 20% CO2 gas mixture. variants[0].relationship: Original strain-specific instruction, separate from the base recipe; no measured growth association is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T03:35:49.362734+00:00; capture SHA256=8441671f114af61c997ae71ee4bf7c9e200d1fdf45764fe120e6a0709b35df80. Additional exact excerpt: For DSM 16661, DSM 21157: Omit growth-stimulating factors and pressurize vials after inoculation to 2 bar with sterile 80% H2 and 20% CO2 gas mixture. variants[0].modifications: Original strain-specific instruction, separate from the base recipe; no measured growth association is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T03:35:49.362734+00:00; capture SHA256=8441671f114af61c997ae71ee4bf7c9e200d1fdf45764fe120e6a0709b35df80.

Provenance