desulfurobacterium_atlanticum_medium

Overview

External ontology

mediadive.medium:829a (source link unavailable) — DESULFUROBACTERIUM ATLANTICUM MEDIUM

Applications

• Microbial cultivation

pH

6.0

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium829a.pdf The inspected original 829a has no final-volume heading. Its 1020.5 ml listed liquid-input sum and the API's 1020 ml volume do not establish a measured denominator. The stock's 250 ml bottle is equipment, not final volume. Full exact-source recipe and stock/preparation tables were inspected. Reconstructed nodes retain explicit source scope; complete prior ingredient/stock assertions, including copied LB mappings and stage conflations, are archived in append-only history. A title alone is not the basis for removal. Unchanged annotations are preserved, not recertified; no new organism-growth association is added.

Composition

flowchart LR
    CultureMech_001979["desulfurobacterium_atlanticum_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001979 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001979 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001979 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KCl["KCl"]
    CultureMech_001979 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NH4Cl["NH4Cl"]
    CultureMech_001979 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_MES__2__N_morpholino__ethane_sulfonic_acid_["MES [2-(N-morpholino) ethane sulfonic acid]"]
    CultureMech_001979 --> ing5_MES__2__N_morpholino__ethane_sulfonic_acid_
    CHEBI_39005["CHEBI:39005"]
    ing5_MES__2__N_morpholino__ethane_sulfonic_acid_ -.-> CHEBI_39005
    ing6_KH2PO4["KH2PO4"]
    CultureMech_001979 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_001979 --> ing7_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing8_Distilled_water["Distilled water"]
    CultureMech_001979 --> ing8_Distilled_water

Ingredients (9)

Preparation

1. Dissolve ingredients (except thiosulfate and sodium sulfide), adjust pH to 6.0, boil medium for 1 min, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add sodium thiosulfate from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Reduce medium with a sterile, neutralized solution of sodium sulfide prepared under 100% N2 gas . Adjust pH of complete medium to 6.0, if necessary.
2. After inoculation pressurize cultivation vessels to 2 bar overpressure using sterile 80% H2 and 20% CO2 gas mixture.

Solutions

Target organisms

No target organisms are recorded.

Provenance